About Andrey Name Dr. Andrey Turchinovich Business Address: German Cancer Research Center, Neuenheimer Feld 581, D-69120, Heidelberg, Germany Business Phone: +49 (0) 622 42 1461 E-mail: a.turchinovich@dkfz.de Education: 2004 October – 2008 April: PhD in neuroscience, International Graduate School of Neuroscience, Ruhr-University Bochum, Germany. Grade: Magna cum Laude. 1998 September - 2003 June: Diploma in Biology (M.Sc), Department of Natural Sciences, Novosibirsk State University, Novosibirsk, Russia; GPA: 4.9/5.0. Graduated with Honors. Research Experience: 2008 August – present time: Postdoctoral Researcher, Molecular Epidemiology Department, German Cancer Research Center, Heidelberg, Germany. 2004 August – 2008 June: Doctoral student, Department of Neuroanatomy and Molecular Brain Research, Ruhr-University Bochum, Germany. Scientific advisors: Prof. Dr. Rolf Dermietzel, Prof. Dr. Ulf Eysel. Dissertation: Functional studies of the gap junction protein connexin43 in vitro and in vivo using RNA interference. 2003 June – 2004 August: Junior Research Fellow, Department of General Plant Genetics, Insitute of Cytology and Genetics, Novosibirsk, Russia. Work on expression of recombinant medical proteins in E.coli and tobacco plans. 2001 September - 2003 June: Diploma research practice, Department of General Plant Genetics, Insitute of Cytology and Genetics, Novosibirsk, Russia and Group of Pharmacogenomics, Institute of Bioorganic chemistry, Novosibirsk, Russia. Principal Investigators: Dr. Elena Deineko, Dr. Maxim Filipenko. Diploma: Creation of transgenic tobacco plants for the production of human interleukin-18. Fellowships In 2004 – 2007: Research fellowship from the International Graduate School of Neuroscience, “From molecules to cognition” program, Ruhr University Bochum, Bochum, Germany. Methods: • Recombinant DNA techniques (molecular cloning in E.coli) • Mammalian cell cultures (neurons, astrocytes, stem cells, cell lines), plasmid DNA tranfection (transient and stable), analysis of gene expression • High-Throughtput RNAi screening; individual siRNA transfection in primary cells and cell lines. • In vitro functional studies using RNAi (cell proliferation, migration, adhesion and apoptosis assays). • siRNA delivery in vivo (retina) • microRNA isolation from cells and blood plasma, TaqMan miRNA Low-Density arrays, individual TaqMan miRNA asssays • Immunocyto(histo)chemistry (cells in culture, cryosections and slices), confocal microscopy. • PCR, Real Time RT-PCR (different platforms), single cell RT-PCR • DNA sequencing (Sanger, Auto ABI310) • DNA, RNA and protein (1D, 2D) gel electriphoresis. • Immuno-enzime assays (Western blot, ELISA) • Southern blot, Northern blot analysis • Proteins expression in E.coli, purification and refolding from inclusion bodies • Affine chromatography of proteins; co-immunoprecipitation. • Polyclonal antibody production (rabbit) • Animal (mouse) handling. Intravitreal injections • Tobacco plants handling, agrobacteria-mediated gene transfer in tobacco. Practical courses: „Practical Course on High-throughput RNAi” at BioQuant, Heidelberg. February 8-12, 2010 “Microarray data analysis with CHIPSTER” at German Cancer Research Center. June 10, 2009 Patents: Turchinovich A, Deineko E, Shumny V, Filipenko M, Khrapov E, Sennikov. «Recombinant plasmid DNA pBi101-IL18 encoding the synthesis of human interleukin-18 in transgenic plants» // Patent №2302460 from 10.07.2007. Publications: Olk S*, Turchinovich A*, Grzendowski M, Stühler K, Meyer HE, Zoidl G, Dermietzel R. Proteomic analysis of astroglial connexin43 silencing uncovers a cytoskeletal platform involved in process formation and migration. Glia. 2010 Mar;58(4):494-505. * - equal contribution Diykov D, Turchinovich A, Zoidl G, Hoffmann KP. Elevated intracellular chloride level in albino rat visual cortex neurons is mediated by Na-K-Cl co-transporter // BMC Neurosci. 2008 Jun 30;9(1):57. A. A. Turchinovich, E. V. Deineko, M. L. Filipenko, A. A. Zagorskaya, E. A. Khrapov, E. A. Filipenko, S. V. Sennikov, academician V. A. Kozlov and academician V. K. Shumny. Transgenic tobacco plants producing human interleukin-18 // Doklady Biochemistry and Biophysics. 2004 Mar-Apr;395:104-7. O. P. Slyudkin, V. K. Khlestkin, A. Ya. Tikhonov, D. Yu. Naumov, N. V. Izarova, and A. A. Turchinovich. Palladium(II) Complexes with 1,2-Bis(alkoxyamine)s // Russian Journal of Inorganic Chemistry. 2002 Vol. 47, No. 1, P. 60-67. Languages: Russian native speaker, English fluent, German basic

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