We have all seen "fat bloom", that unwelcome white layer that occasionally forms on chocolate. It is harmless but Europe once banned ugly fruit so cosmetics are clearly important to them and for that reason Nestlé and the Hamburg University of Technology got the DESY synchrotron's high brilliance X-ray source PETRA III on the case.
Fat bloom can form when liquid fats, such a cocoa butter, migrate through the chocolate to the surface and crystallise there.
"This can happen when liquid chocolate cools down in an uncontrolled manner and unstable crystals form, for example. But even at room temperature, a quarter of the lipids contained in chocolate are already in a liquid state," says lead author Svenja Reinke of the Hamburg University of Technology.
Liquid fillings or ingredients such as nougat can accelerate the formation of fat bloom.
With DESY's bright X-ray source PETRA III scientists could follow the migration of liquid fat (yellow) through chocolate live for the first time. Credit: Svenja Reinke/Hamburg University of Technology
The longer chocolate is stored and the higher the storage temperature, the more likely fat blooming is observed. This is why the white coloration of the product surface is often taken to be a sign of old or spoiled chocolate. "Even though fat bloom does not actually constitute any deterioration in the quality of the product, the visual alteration associated with it can lead to a large number of consumer complaints," points out Prof. Dr.-Ing. Stefan Palzer, from the food company Nestlé. "This is why fat bloom continues to be one of the most important quality defects in the confectionary industry."
With the help of DESY's x-ray source PETRA III, the scientists have now been able to observe the underlying processes in real time. To do so, they investigated the behavior of different mixes of the main components of chocolate, which are cocoa, sugar, milk powder and cocoa butter. These technical chocolate samples were ground to a fine powder so as to speed up the processes and then X-rays were shone through them.
"The technology used to examine the samples shows us both the fat crystals and the pores inside the product, down to a scale of a few nanometers," explains Prof. Dr.-Ing. Stefan Heinrich from TUHH, leader of the investigation.
In order to study the migration of fats, the researchers also placed a few drops of sunflower oil on each of their samples and observed what happened.
"First of all, wetting takes place within seconds. The oil penetrates very quickly into even the smallest pores, probably through capillary action," Reinke describes their observations. Secondly, the liquid fat alters the internal structure of the chocolate. "Over a period of hours, the liquid fat dissolves additional crystalline lipid structures, which makes the entire structure of the chocolate softer. This in turn increases the migration of lipids."
The dynamics of the development of fat bloom have so far been unknown, so that this study adds to the findings of previous experiments on the crystalline structure as one major factor influencing fat bloom.
"For the first time, we have been able to track in detail the dynamic mechanisms that lead to the creation of fat bloom," explains DESY scientist Dr. Stephan Roth, head of the P03 beamline at PETRA III, at which the experiments were conducted. "The method used is known as small-angle X-ray scattering and is precisely adapted to real-time investigations of this kind, and to observing the structural changes caused by the moving lipids. The joint study has supplied important information as to how we can study structural changes in such 'everyday' multi-component systems."
The observations allow the food industry to develop concrete approaches for reducing fat bloom. "One consequence might, for example, be to reduce the porosity of the chocolate during manufacture, so that the fat migrates more slowly," explains Reinke. "Another approach is to limit the amount of fat that is present in a liquid form by storing the product in cool, but not too cold, conditions. 18 degrees Celsius is ideal."
Chocolate is very sensitive to fluctuations in temperature.
"Just a few degrees make a big difference," Reinke notes. "At 5 degrees, basically, all cocoa butter is solid; and above about 36 degrees everything is liquid."
On top of this, the type of crystals in the chocolate plays an important role.
"Cocoa butter crystallizes in six different crystal forms," explains Reinke. "The amount of fluid also depends on the form of the crystals."
Manufacturers can also limit fat bloom by controlling crystallization.
Citation: Tracking structural Changes in Lipid Based Multicomponent Food Materials due to Oil Migration by microfocus Small-Angle X-ray scattering; Svenja K. Reinke, Stephan V. Roth, Gonzalo Santoro, Josélio Vieira, Stefan Heinrich, Stefan Palzer; ACS Applied Materials and Interfaces, 2015; DOI: 10.1021/acsami.5b02092