Researchers from Iowa State University have identified a factor that promotes the development of antibiotic resistance in a bacterial pathogen. The study explains that Mfd, a protein involved in DNA transcription and repair, plays an important role in the development of fluoroquinolone resistance in Campylobacter, a bacterial pathogen commonly associated with food poisoning in humans.
Development of antibiotic resistance in Campylobacter, especially to fluoroquinolone (a broad-spectrum antimicrobial), compromises clinical therapy and poses a public health threat. Previous studies have revealed that Campylobacter is highly mutable to antibiotic treatment and the number of fluoroquinolone-resistant Campylobacter is greatly increased in many regions of the world. But it has not been clear why Campylobacter is able to mutate at such a high frequency.
Using various molecular tools, the research team from the College of Veterinary Medicine, led by Dr. Qijing Zhang, has found that Campylobacter increases the production of Mfd in the presence of a fluoroquinolone antibiotic. Elimination of Mfd from Campylobacter resulted in 100-fold reduction in the rate of emergence of mutants resistant to fluoroquinolones. Without Mfd, the development of fluoroquinolone-resistant Campylobacter under antibiotic treatment is significantly reduced.
These findings have uncovered a previously unrecognized role of Mfd in promoting mutations conferring antibiotic resistance. Despite its importance, Mfd is not the only factor influencing the mutation frequency and future studies will be needed to determine how Mfd increases the emergence of antibiotic-resistant mutants.
CITATION: Han J, Sahin O, Barton Y-W, Zhang Q (2008) Key Role of Mfd in the Development of Fluoroquinolone Resistance in Campylobacter jejuni. PLoS Pathog 4(6): e1000083. doi:10.1371/journal.ppat.1000083