AYURVEDIC MEDICINES: SOME POTENTIAL PLANTS FOR MEDICINE FROM INDIA.
AYURVEDIC MEDICINES: SOME POTENTIAL PLANTS FOR MEDICINE FROM INDIA. Ashwini Kumar Former Alexander von Humboldt Fellow, Insitut fur Pflanzenernaehrung der Justus Liebig Universitat, Giessen. Germany and Former visiting Professor , Research Institue for Wakan Yaku, Toyama Medical and Pharmaceutical University, Toyama, Japan. Present address: Energy Plantation Demonstration project and Biotechnology Center. Department of Botany, University of Rajasthan Jaipur,302004 ( Rajasthan) India. Email : firstname.lastname@example.org Phone : 00 91 141 654100 Fax 00 91 141 565905 Abstract: With the changing pattern of life style most of the diseases are now becoming lifestyle diseases. The traditional system of medicine based on ancient culture are primarily concerned with building the body strength which can help in healing the ailments and these systems rely largely on the nature cure. Ayurvedic system has described a large number of such medicines based on plants or plant product and the determination of their morphological and pharmacological , pharmacognostical characters can provide a better understanding of their active principles and mode of action. Introduction: Contribution of the traditional medicine to human health in the 21st Century is of paramount importance. A meeting of International Forum on Traditional Medicine held recently (1999) at Toyama Medical and Pharmaceutical University, Toyama, reviewed the potential of traditional medicines. WHO acting director Xhang emphasized that with the changing pattern of life style most of the diseases are now becoming life style diseases. Natural medicines improve the inner strength of the body. The use of traditional medical system has attracted so much attention that an International health Center has been opened in July in Toyama prefecture (Province) in Japan. Some of the oldest traditional medical systems include Chinese, Ayurvedic, Unani, Japanese and recently added homeopathy and chiropathy that is also around 200 years old. The use of traditional medicine includes (i) medication by use of medicinal plant, minerals, animal material and (ii) Non medication: acupuncture and yoga. Complementary medication includes – acupuncture, herbal treatment, manual, spiritual and dietary treatments. Toyama hospital utilizes vast amount of Chinese, Japanese and Ayurvedic medicine. Detailed studies in the areas of pharmacognosy and pharmacology are under progress ( Annual report, TMPU, Toyama). Besides this the Research Center for Ethnomedicines with its Museum of Materia Medica is one of its own kind in the world under Professor Watanabe. Dr. Komatsu provides wealth of information for all the scientists engaged in the field all over the world. This includes identification, molecular characterization at DNA sequence level, chemical characterization, biotransformations and studies on effects on microorganisms to direct application in the hospital. To give entire description will be attempted in another paper. Here a brief attempt is made to identify common goals of research in India and Japan, with an object to attract attention of the workers to the great potential that the great bio-diversity of Indian subcontinent and wealth of Ayurvedic literature has to offer for future development of traditional medicines. However detailed future investigations are needed in this area to exploit the unexplored or poorly explored plant materials. These traditional medicines have found practical application at clinical level in TMPU and over hundred cases of fissure have been cured in the hospital using a special thread prepared from latex of Euphorbia spp., thor of India (Euphorbia sp) , haldi powder Curcuma longa and some herbal ingredients. The Euphorbia sp is a plant of desertic region of India and different parts of the world. A large number of energy yielding desertic plants of India used in Ayurvedic system have great potential as Ayurvedic medicine . Negative environmental effects of current agricultural practices, such as emission of greenhouse gases, nutrient leaching, decreased soil fertility, and erosion, may be reduced when traditional annual food crops are replaced by dedicated perennial energy crops and medicinal plants. As they are able to grow and produce valuable products under dessert conditions they have great potential for covering the global desert areas into green belts leading to environmental improvement on one hand and providing valuable Ayurvedic crude drugs in addition to supplementing the bio-energy resources as renewable fuels. However detailed studies on their pharmacognostical characterization and determination of chemical products obtained from them are lacking. Some of the investigations indicated their potential use in Human immuno deficiency (HIV) diseases ( Hattori et al 1995). Such bio-energy plants have not been explored in depth. Here attempt shall be made to provide brief out look of the Indian scene and highlight some of the work being carried out at our place in Rajasthan along with the possible impact assessment for desertic plants for future research strategies. Among the desert plants the value of Aloe vera(L.) was recognized more than 3000 years ago when the Egyptian and Greek civilizations used its extract for skin burns, cut and wounds on the skin surface and found that it had a wonderful healing effects on skin. It is claimed that even 3rd degree burn can be cured and healed by Aloe vera. The chemical compounds like Aloein, resins, mixture of polysaccharides containing pectic acid are present. Modern investigations indicate that extracts of Aloe vera act on the dead epithelial cells of skin, aiding their removal from the surface and stimulating the growth of new cells. Thus Aloe is providing to be a great gift of traditional medicine for protecting the smooth skin of human beings especially when radiation damage of human skin has assumed alarming situation due to stratospheric ozone depletion. Fresh juice of leaves are also used in liver and spleen troubles and also for eye troubles, found useful in X-ray burns, dermatitis, coetaneous and other skin disorders. In India, Egypt and Sudan around 70 percent of the rural people use traditional medicine. Similar situation exists in a large number of developing countries. In India and China 60 percent of the people affected with cholera and malaria are treated with herbal medicines. In these countries the market for traditional medicines is US $ 500 million while Western type medicine account for only US $ 300. In Singapore 50 percent and in Australia 60 percent of population uses alternative medicine. Around 17,000 herbal products are registered in these countries. In Belgium 40 percent contemporary and 84 percent home medicines and 74 percent acupuncture medicine is utilized. In France 50 percent of the people take advantage of complementary medicine. In Germany 10,000 to 13, 000 alternative medical practitioners are thriving well and 75 percent of them utilize complementary medicines. 77 percent of pain clinics utilize acupuncture. In UK 90 percent of the complementary medical practitioners utilize osteopathy and acupuncture. In US where in 1990 only 30 percent of the people were utilizing complementary medicines in 1997 it grew to 40 percent. Ayurvedic system of Medicine: Ayurveda is an offshoot of Atharva veda written over 3000 thousand years ago. The Charak and Sushruta describe a large number of crude drugs and a large part of them has origin to plants. However though some part of it has been translated from Sanskrit to Japanese and Japan Society of Ayurveda under Professor Dr. Namba is very active in this field but many of the crude drugs described remain to be identified to its plant source in botanical terms and Institute of Traditional Medicine is the prime center for understanding the nature and morphology of crude drugs of Ayurvedic origin and their identification to the plant level. The personal communication with Professor Watanabe and Dr. Komatsu during my stay at Wakan Yaku as visiting Professor has contributed to the stimulation of such studies back home and some of the important findings are presented here. During my stay here I have worked on Nepalese crude drugs with support and guidance from Dr. Komatsu and other members of this institute. The basic philosophy of Ayurveda considers that man is an inseparable part of the universe. The human body mind and spirit continuum is an integral whole and the individual is also linked to the family, society, environment and ultimately the universe. The definition of health is that “ It is state of complete psychosomatic equilibrium. It does not mean only absence of diseases but a state in which the mind, senses and spirit are pleasant and active. “ That agrees with the definition of WHO “Health is a state of complete physical, mental and social well being and not merely the absence of disease or infirmity” India with its varied climate soil and agro-ecology possesses immense plant diversity, with over 15,000 species of higher plants. Both our Indian civilization as well as our diverse tribal heritage have gone a long way in conserving the wild weedy species, native land races and primitive cultivars ( Fig.1) . The Indian gene center is endowed with rich flora especially with regard to several less known yet economically important plants, ca 160 cultivar species of economic plants, plus 56 species of lesser known cultivated food plants. Further there are ca 320 species of wild and weedy economic types ( Paroda 1979; Arora and Nayar, 1984 ; Kumar, 1998). The unutilized and underutilized resources: Out of 2,50,000 plant species only 10,000 or so have been exploited during the course of human civilization. A large number of hydrocarbon yielding plants are able to grow under semi arid and arid conditions and they also produce valuable hydrocarbons ( upto 30 percent of dry matter) which could be converted in to petroleum like substances and use as fossil fuel substitute. They are rich in triterpenoids which are constituent to important drugs against HIV. The potential plants: Certain potential plants were selected and attempts were made to develop agro-technology for their large scale cultivation (Kumar et al, 1995, Kumar,1998, Kumar, 2000). A 50 ha bio-energy and medicinal plants cultivation demonstration center has been established in the campus of the University of Rajasthan to conduct the experiments on large scale cultivation of selected plants with the objective of developing optimal conditions for their growth and productivity, besides conserving the bio-diversity. Plantation of laticiferous plants and desert plants can be carried out, it could also lead to reclamation of marginal land that has already been abandoned in developed as well as developing countries. India alone has over 144 million-hectare of marginal land which is about half of the total geographical area of the country. Touched only marginally by the green revolution Africa suffers not only a dramatic nutritional problem but also and equally serious and inter linked problem of energy. Increasing scarcity of fuelwood, desertification, lack of water, food and medicines , excessive urbanization all are closely interdependent and rich biodiversity in developing countries has remained unutilized and underutilized for want of proper investigations . There are sure to be opportunities for Biomass of the medicinal plants in the south as well as in the North in wet climates and in dry ones but they will respond to very different schemes and strategies. There is not going to be a single unique recipe rather multiplicity of solutions depending on climate, soil, availability of land traditions social and economic conditions. Technological improvements should lower production costs but they are unlikely to obtain significantly higher yields, as chemical and energy inputs must be reduced. The transformation of Biomass into useful energy products and medicinal compounds may however involve on site industrial operations that could absorb at least part of the surplus man power. As far as research is concerned we are all aware of the important progress being made in agricultural biotechnology. Genetic engineering for example is increasingly applied to crop plants for improving resistance to pests and diseases, for providing more favorable crop composition. There is a whole universe of possibilities in the use of advanced biotechnology to improve plants and processes. The natural medicine from plants has enormous possibilities for new and more effective means for curing the modern day ailments. Natural resource: A detailed survey on the weeds on wastelands yielded valuable data about the first colonizers. Total land area of Rajasthan is 3,42,239 sq km out of which 45.25 percent is characterized as wasteland. Large portions of this land were productive at a given time and due to man made deforestation, cattle pressure, water and wind based soil erosion, improper water management, they have turned out to be wastelands. ( Kotia and Kumar, 2001a). A large number of such weeds have the medicinal value. Out of the total weeds around 50 weeds having important medicinal values while other produce related compounds. These regions are rich in bio-diversity and weeds were collected from different regions of the developing wastelands. ( Kotia and Kumar, 2001b) . A detailed survey was carried out in different parts of Rajasthan and some of the medicinally important plants of Rajasthan are listed by Ajanta and Kumar, ( 2001a) . Some of the Medicinal plants found in wild in the forests of Rajasthan include: _______________________________________________________________________ Table1. List of Medicinal plants of Rajasthan. ___________________________________________________________________ Plant species : Local name 1. Asparagus racemosus satavari 2. Chlorophytum arundinaceum Safed musli 3. Curculigo orchioides Kali Musali 4. Solanum surattense Kantkari 5. Boerhaavia diffusa Santhi, 6. Hamidesmus indicus anantmool 7. Sida cordifolia Bala 8. Holarrhena antidysenterica Indrajo 9. Curcuma aromatica Vanhaldi 10. Oroxylum indicum Shyonaka 11. Balanites aegyptiaca hingot 12. Withania somnifera ashwagandha 13. Aegle marmelos Bael 14. Cassia fistula Amaltas 15. Gymnema sysvestre gudmar 16. Terminalia arjuna arjuna 17. Butea monosperma palas 18. Soymida febrifuga rohan 19. Woodfordia fruticosa dhavri 20. Tribulus terrestris gokhru 21. Pedalium murex badagokhru 22. Vitex negundo negad 23. Dyerophytum indicum chhitral 24. Plumbago zeylanicum Chitrak 25. Plantago ovata Isabgol 26. Colocynthes vulgaris Indrayan 27. Adhathoda vasica ardusta 28. Allangium salvifolium aankol 29. Caesalpinnia bonducella tas 30. Jatropha curcas ratanjot 31. Eclipta alba bhringraj 32. Aloe barbadensis gwarpatha 33. Mucuna prutita Konch 34. Terminalia bellerica baheda 35. Tamarindus indica imli 36. Azadirachta indica neem 37. Achyranthes aspera aandhijhara 38. Barleria cacrulea bajrandanti s 39. Barleria cristata. Badradanti p 40. Barleria prinoitis bajradanti p. 41. Ocimum americanum bapchii 42. Centella asiatica brahmibuti 43. Datura metel. Dhatura 44. Convolvulus arvensis haranpadi 45. Evolvulus alsinoides shankhpushpi 46. Cassia occidentalis kasaundi 47. Urginea indica Kolikanda 48. Andrographis paniculata kalmegh 49. Helicteres ispara marorphali 50. Tinospora cordifolia nimgiloy. Calotropis procera (Ait.) R.Br. (Akanda, Alarka, Aak) : The plant is one of the important number of traditional herbal medicine in every home of India. Traditionally the leaves of aak are warmed and tied around any body organ in pain. It is practically useful in backache and in joint pains warm leaves also relieve from stomachache if tied around. Inhalation of burnt leaf cures headache. The traditional folk healers use the milky latex of aak for several ailments. Leaf latex if applied on fresh cut, stops bleeding immediately. Recent investigations have found that the alkaloids calotropin, calotaxein and uskerin is stimulant to heart. Flowers and roots are used in Ayurvedic medicine. Plant is anthelmintic, the ashes act as an expectorant. The leaves are applied hot to the abdomen to cure the pain inside. Flower is tonic, antisialagogue, appetizer, and stomachache, cures piles and asthma. Flowers are believed to have detergent properties so they are given in cholera. The fresh roots are used as a toothbrush and are considered by pathans to cure toothache.. Alarka is an alternative, tonic and diaphoretic, in large dose emetic. Root bark useful for treating chronic cases of dyspepsia, flatulence, constipation, loss of appetite, indigestion and mucus in stools. Leaves used against guinea worms. Flowers useful in asthma. Seed oil is geriatric and tonic. Green copra given in asthma. Plant used in spleen complaints, rheumatism, epilepsy, hemiplegia, sores, and smallpox and protracted labor. Properties and uses of Alarka are the same as of C.gigantea Calotropis gigantea R.Br. (Arka) : Arka is purgative, anthelmintic alexipharmic,; cures leprosy, ulcers, leucoderma, tumors, piles, diseases of spleen, liver and abdomen. Juice is anthelmintic and laxative; cures piles and kapha. Dried and powdered plant is taken with milk acts as a good tonic. Action is similar to Digitalis on the heart. Root bark and juice have emetic, diaphoretic, alternative and purgative properties. It is used in dysentery and as a substitute for Ipecauantha. It is regarded as a great remedy in syphilitic afflictions and is called “Vegetable mercury”. In intermittent fevers it is used as antiperiodic and diaphoretic. It cures asthma and syphlis. In form of paste applied to elephantiasis. Tincture of leaves use in intermittent fevers. Latex is bitter, heating, oleagenous and irritant, used in combination with Euphrobia neerifolia as purgative. Flowers are sweet, bitter, digestive, tonic, stomachic, anthelmintic, analgesic, astringent; cure inflamations, tumours, kapha and are good in ascites. Jatropha curcas Linn. (Vyagrairanda) : Juice of Vyagrairanda is a well known purgative and is useful in whitlow, convulsions, syphlis, neuralgia, dropsy, anasarca, pleurisy and pneumonia. Root bark is applied externally in rheumatism and is used in sores. Leaves are galactagogue, rubefacient, suppurative, insecticidal and are used in foul ulcers, tumors and scabies, given internally in jaundice. Leaves are locally applied to breasts to increase secretion of milk. Leaves warmed and rubbed with castor oil and applied to boils and abscesses have supportive effect. Decoction of leaves is antidiarrhoea useful in stomach-ache and cough also used for gargle to strengthen gums. Fresh stems are used as toothbrush. Fresh viscid juice flowing from stem is employed to arrest bleeding or hemorrhage from wounds. Stem bark is used for wound of animal bites. Fruit and seeds are anthelmintic, useful in chronic dysentery, urinary discharges, abdominal complaints, anaemia, biliousness, fistula, and diseases of heart. Seeds are acro-nacrotic, poisonous to human beings and cattle and used against warts and cancers, also to promote hair growth. Seed and oil are purgative, more drastic than castor oil. Wood causes dermatitis. Drug is bitter, acrid, astringent and anthelmintic. It serves to cleanse the entire system through its purgative property. It is useful in chronic dysentery, thirst, abdominal complaints, biliousness, anemia, fistula, ulcer, and diseases of the heart and skin. Croton tiglium Linn. (Jamaalagotta, Jayapala) : Jayapala seeds and oil are drastic purgative, diaphoretic, vasicant, vermifuge irritant, rubefaceint and cathartic. Its action is prompt. Croton oil when rubbed on skin acts as a rubefaceint and counter-irritant and vesicant. When administered internally it operates as a powerful hydrogue cathartic. It is found to be very useful in ascites, anascara, cold, cough, fever, asthma, constipation, calculus, dropsy and enlargement of abdominal viscera. It is given only when a drastic purgative is required as in dropsy and cerebral affections like convulsions, insanity and other fevers, attended with high blood pressure. Wood is diphoretic in small doses and purgative and emetic in large doses. Euphorbia hirta Linn. (Dudhi, Cara) : Cara is demulcent, antispasmodic, anti-asthmatic pectoral, anthelmintic and local parasiticide. Plant is chiefly used in the affections of childhood, in worms, bowel complaints and cough, in postnatal complaints, failure of lactation, breast pain. Extract of plant has depressant action and action on cardiovascular system, a sedative effect on mucous membrane of respiratory and unino-genitory tract. Juice of plant is given in dysentery and colic, and milk applied to destroy warts. Plant alkaloid is effective in respiratory system and produces dilation of bronchi. Decoction of plant is used in bronchial affections and asthma. Latex is vermifuge and used in diseases of urinogenitory tract, also used in application for warts. Euphorbia tirucalli Linn. (Vajraduhu, Satsala) :It is useful in biliousness, leucorrhoea, leprosy, dropsy, whooping asthma, enlargement of spleen, dyspepsia, jaundice, colic tumours, and stone in bladder. Milky juice is vesicant, rubifacient. In small doses a purgative but in large doses it is acrid, emetic and counter-irritant; application for warts, neuralgia,rheumatism, toothache, used in asthma, cough and earache, fish poison. Milky juice is applied to itch and scorpion bites. Decoction of tender branches and that of roots is administered in colic and gastralgia. Anti-HIV agents among desert plants: Around 40million people are affected due to Human Immuno-deficiency Virus Globally. During the past decades, a large number of anti-viral screening experiments on medicinal plant extracts have been reported and have led to the selection of several extracts active towards herpes viruses. A promising result of a naturally occurring antiherpetic agent was given by n docosanol ( a natural 22 carbon saturated fatty alcohol) which is undergoing phase III clinical trials in patients. Clinical testing of the topical formulation, or systemic administration of drug suspensions has demonstrated a good therapeutic index, since high doses of n-docosanol do not elicit appreciable toxicity. The findings show that natural products are still potential sources in the search for new antiherpatic agents( Hattori et al. 1995,). Various plant extracts used in Ayurvedic medicine for inhibitory effects on HIV virus have been studied (Hattori et al, 1993 ; Kusumoto et. 1995; Hattori, personal communication). A large number of such plants occur in semi-arid and arid climate of Rajasthan ( Roy and Kumar, 1995) . Acquired immunodeficiency syndrome (AIDS) , the great pandemic of the second half of the 20th Century, is still a threatening disease world wide. Many research approaches are currently aimed at developing novel agents to arrest the replication of HIV through various targets. These may include the inhibition of reverse transcriptase (RT), protease (PR), membrane fusion and integrase. HIV PR enzyme has been demonstrated to play an essential role in viral replication (Meek et al. 1990). It is considered as potential target for anti-AIDS therapy, as the inhibition of this enzyme produces immature, non-infectious virions ( Mous et al. 1988; Huff, 1991; Robins and Platter, 1993). A range of HIV PR inhibitors have been designed and applied in clinical trials such as Sanqunavir, Ritonavir and Indinavir. However, the development of drug resistance by virus irrespective of the target, remains as an overwhelming problem in AIDS chemotherapy ( El Farrash, et al. 1994). Thus there is great need to search for and develop new and different anti-HIV candidates from plants and natural products are of considerable importance. In search for anti-HIV active agents from natural products, many attempts at screening traditional medicines have been made ( Chang and Yeung, 1989; Otake, et al. 1995; Wan et al. 1996). However Indian and other tropical region plants with their vast diversity, have not been investigated for their antiviral activity. Hussein et al ( 1999) investigated forty eight methanol extracts from Sudanese plants which were screened for their inhibitory activity on viral replication. Nineteen extracts showed inhibitory effects on HIV –induced cytopathic effects (CPE) on MT-4 cells. The extracts were further screened against HIV-1 protease (PR) using an HPLC assay method. Of the tested extracts, the methanol extracts of desert plants Acacia nilotica (bark and pods), Euphorbia granulata ( leaves) , Maytenus senegalensis (stem – bark) and aqueous extracts of A. nilotica (pods) and M. senegalensis (stem-bark) showed considerable inhibitory effects against HIV-1 PR (Hussain et al. 1999) . Some of the plants of Sudan are common with Indian dessert region of Rajasthan and generally they grow on the wastelands. They have potential use as bio-energy plantations ( Kumar et al. 1995, Kumar, 1998 ) . However a large number of them are used in the medicines of Ayurveda. They were also found effective against HIV-1. ( Hussein, et. al. 1999) . A list of potential plants of this region is given here in table no. 1. However these plants have not been studied in detail and there is need to study them for their medicinal properties including anti- HIV properties. Some of the active principles against anti-HIV are triterpenoids which are abundant in laticiferous plants of Rajasthan. Besides Ganoderma sp is very frequently met in Rajasthan attacking trees. Ganoderma lucidum has been described to contain triterpenes which have inhibitory effects against HIV-1 protease ( Min et al. 1998) . Besides this several other plants like Abrus precatorius L. (Leguminosae) Chao-mei, Nakamura and Hattori, 1998) , Datura stramonium L., Balanites aegyptiaca (L.) Delile etc commonly found in Rajasthan show anti HIV activity (Kawahata et al. 1996) . an established is widely distributed in Rajasthan and allover India. Its seeds in China have been used as an insecticide and for skin diseases since ancient times . A detailed survey of medicinally important plants have been carried out and important trees, shrubs herbs have been listed and their characters studied in the several publications from our laboratory. They included drugs for cure of urinary tract infection (Ajanta and Kumar, 2000b) anti-depressant herbal drugs ( Ajanta and Kumar, 2000c), medicines for skin diseases ( Shivani and Kumar, 2000), anticancer drugs (Sharma and Kumar, 2000); anti-diabetic drugs ( Ritu and Kumar, 2000). Herbal drugs of Leguminosae from Rajasthan have been studied ( Sapna and Kumar, 2000). Herbal crude drugs for anti-malaria ( Anita and Kumar, 2001); anti-paralytic ( Vandana and Kumar, 2001). Besides this herbal crude drugs for cure of hepatic diseases ( Santosh and Kumar, 2001) and diseases of digestive system ( Mamta and Kumar, 2001) have been studied for their characters and investigations on their morphological and pharmacognostical characterization is in progress. Conclusion: The sustainable land utilization in ecologically fragile climate of semi arid and arid regions, has to be guided by the principal of optimal utilization of resources. It is a matter of great interest that a large number of plants of the arid and semi-arid regions of the world are effective as anti-HIV agents. They are also used in variety to herbal and traditional medicines as listed in this paper. Our previous work on their bio-energy production potential, if combined with their crude drug potential could yield bio-fuels on one hand and valuable crude drugs on the other hand. However a large number of tropical plants have not been studied in detail for their chemical constituents, pharmacological properties of the extracts, and their pharmacognostical characterization and DNA sequencing etc. If a joint collaboration could be established in this direction, valuable information could be generated with wide ranging practical applications. This could also provide alternative land use pattern for the rural poor thriving on marginal lands on one hand and help in eco-restoration on the other hand. The use of bio-energy plants in the herbal crude drugs has great potential and detailed inventions are planned with the help and cooperation with the help from different agencies. This paper provided a brief outline of the work in the area for future suggestions and improvement. Acknowldegement: I am deeply indebted to Professor Watanabe, Director of the Research Institute of Wakan Yaku for providing me an opportunity to work as visiting Professor at the Institute and Dr. Komatsu for constant encouragement support during my stay and work at the TMPU. Toyama.The keen interest and encouragement by Professor Dr. Neumann during the course of this work is gratefully acknowledged. Literature cited. Mishra, A and A. Kumar ( 2001a) Medicinally important trees of Rajasthan. Int. J. Mendel. 18: 37-38. Mishra, A and A. Kumar. (2001b )Studies on Ayurvedic crude drugs for the cure of urinary tract stones.18 (1-2): 41-42. Mishra, A and Ashwani Kumar. Searching for anti-depressant crude drugs in ayurvedic system of medicine. Int. J. Mendel. 17 (3-4) : 91-92. . 2001c Yadav, A and A. Kumar ( 2001)Studies on anti-malarial drugs used in Ayurvedic system. Int J. Mendel Int. J. Mendel. 18(1-2):29-30. Kumar, A. , S, Johari, and S. Roy. 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