First for anyone who doesn't know, NASA’s perseverance rover is currently collecting small samples of rock and leaving them on the surface in tubes on Mars. NASA want to return them some time around 2033 and they plan to return its Mars samples of rock and some dust / soil to biosafety level 4 facilities (BSL-4). But the ESF in 2012 said we have to contain ultramicrobacteria from Mars and a BSL-4 can’t do that.

See longer version of this post with extra graphics and explanations for non experts.

As Carl Sagan said:

The likelihood that such pathogens exist is probably small, but we cannot take even a small risk with a billion lives.

Carl Sagan, 1973, The Cosmic Connection - an Extraterrestrial Perspective.

I’m posting here as I got no replies from NASA and so far all the experts I contacted either didn’t reply or just referred me back to NASA. Comments are here,. The public comment period ended at 11.59 pm Eastern time, 19th December .

my last comment here which gives a short summary of the issues in 14 points plus the details in attachments:

. Robert Walker, Dec 20, 2022, I recommend this draft Environmental Impact Statement is stopped, and a new one prepared after doing the necessary size limits review, and fixing whatever led to its many errors....

If this does get through NEPA I expect the presidential directive to stop it, which happens at the end of the legal process and must look at anything if it can be reasonably expected to result in domestic or foreign allegations of major or protracted effects which this surely will be. But it is far better to get it on the right track now than to go through all that.

Video for this blog post here (uses earlier edit of this post)

Click to watch on Youtube: Why doesn't NASA respond to public concerns on its samples from Mars environmental impact statement?

This blog post goes nto much more detail, and also links to my annotations of the draft EIS and the academic preprint analysing the EIS.

. NASA - Your Samples From Mars Need A Better Than Biosafety Level 4 Facility - NOT Designed To Contain Even Earth's Tiniest Cells


Dear NASA. former and current planetary protection officers, astrobiologists, flight engineers, anyone interested and the general public.

My background is that for the last 2 years I've been working on a paper I hope to submit to astrobiology journals specifically on planetary protection for NASA's Mars sample return mission. I did an early draft as a presentation on a Mars sample return that I prepared but didn’t present when I gave this presentation about Enceladus and Europa at a small astrobiology conference in Oxford, in 2015.

. "Super Positive" Outcomes For Search for Life In Enceladus and Europa Oceans - Robert Walker

So I'm reasonably familiar with the literature.

So I was astonished when NASA's Environmental Impact Statement didn't cite this recommendation from the European Space Foundation from 2012. This is the most recent Mars sample return study. How can they not know about it? I’m baffled.


… the ESF-ESSC Study Group recommends that values on level of assurance and maximum size of released particle are re-evaluated on a regular basis.

The release of a single unsterilised particle larger than 0.05 microns is not acceptable under any circumstance

WELL BELOW Biosafety 4 limits - we don’t have ANY air filters yet that can do this

Page 48 of 2012. Mars Sample Return backward contamination–Strategic advice and requirements

NASA plans to use a BSL-4 laboratory to contain rocks and dirt returned from Mars by 2033 by its Environmental Impact Statement, but that is basedon the science of 1999. The science of 2012 says we have to contain ultramicrobacteria at 0.05 microns WELL BEYOND the capabilities of a BSL-4 indeed the technology doesn’t exist - it is rare to need to contain such very small organisms or particles.

I looked at the literature, nobody knows how to contain such small particles confidently. Maybe it can be done but a decade later nobody is trying as far as I can tell. There aren't many situations where you need to contain such tiny particles in air.

The ESF also recommended that their size limit and their required level of assurance needs to be re-evaluated on a regular basis and NASA haven’t even used the 2012 limit.

Instead they plan to use a standard BSL-4 facility - this recommendation is based on the scientific understanding of 1999 if you trace it back to its original sources. That’s more than two decades old science.

I have tried contacting NASA about why they aren’t using the most recent European Space Foundation study, and got no response including via public comment on their proposals in May.

. Public comment by Robert Walker, May 16, 2022

They still don't mention it in the draft EIS which they published in November.

The public comment period ends at 11.59 pm Eastern time, 19th December and after that there is no more chance for public comment as far as I can tell, and they expect it to be completely finished under the fast NEPA process in spring / summer 2023.

But it is kind of weird the argument. All the way through they miss out important studies and misrepresent the sources they do cite. They also contradict themselves pretty much.

It is just so unlike NASA and I don't see how it got through internal review but I have to comment on what I can see there. It’s not for me to speculate how it happened.

I've also tried emailing many people, about a dozen so far including experts in astrobiology or planetary protection. So far most haven't replied; others reply just saying to ask NASA which is no use as NASA aren't responding. There are many in the public who have raised concerns of one sort or another. NASA have a section in the draft EIS where they say they responded to public comments, but they don't mention this concern about BSL-4 facilities not being sufficient. There is no response to major concerns raised by the public.

It is really quite extraordinary. Their basic argument is that they claim:

  • Credible evidence says that Mars has been uninhabitable for millions of years
    (their cite is about searches for local currently habitable regions in a seemingly uninhabitable planet)
  • If there is life on Mars, they say it can't get to Jezero crater
    (why do we need to consider this if Mars is uninhabitable? And they do this by citing the 2014 MEPAG survey and not the second MEPAG study in 2015, SR-SAG2 which revised all the relevant MEPAG conclusions - the second study draws attention to biofilms a microbe can use to make extreme environments more habitable, says regions that seem uninhabitable from orbit may have localized habitats, and warns that we don't know if terrestrial life can be transported in dust storms - also their MEPAG cite is about forward contamination by terrestrial life, so isn't even about the potential capabilities of any martian life which would have evolved to conditions on Mars and the dust storms over billions of years.)
  • If there is life in Jezero crater, they say it can get here faster and better protected in meteorites
    (their cites are about rare cases in panspermia such as b subtilis, a very hardy organism, none of their cites say life could get here faster and better protected in a meteorite, and what matters for planetary protection, unlike panspermia, the theory that life could be transferred between planets, is life that can't get here, metaphorically the starlings are the invasive species in the Americas because they can’t get across the Atlantic while barn swallows are no problem because they can - and you have microbes too like “Didymo” which is an invasive species in New Zealand because it can’t cross oceans and can’t even get from one freshwater lake to another in New Zealand without help from humans. There could easily be microbes that could get from one briny seep on Mars to another in the dust storms that could never get to Earth on a meteorite while it could use a sample tube with a small amount of martian atmosphere sealed in like a miniature spaceship)
  • Because of all this they say that there is no significant risk of environmental effects and nothing needs to be considered about human health except to handle the samples like any other toxic chemical or infectious disease
    (the NRC study from 2009, which they DO cite, warns that though the risk of large scale effects on the environment or human health is likely low, it is not demonstrably zero, and it warns against using the very meteorites argument that they use)
  • That we have to return the samples to Earth for "safety testing" - this rules out any possibility of a pre-sterilized sample return (sterilized before it gets to Earth) which they don't even mention
    (actually the permitted biosignatures per gram of rock sample or regolith for the returned rock samples are so high that any tests for life are guaranteed to detect biosignatures from Earth - NASA think they achieved a reduction to 0.7 nanograms per biosignature per gram for the most abundant biosignatures - so the safety testing is useless because their cite says that the only way we can check for safety is to check if there is martian life there since we can’t predict effects of introducing novel life to Earth - and again - why are we doing safety testing if they already know Mars is uninhabitable, know it can't get to Jezero crater from elsewhere on Mars and know it can get back to Earth faster and better protected in a meteorite?)

To put that in context one nanogram is enough for 10,000 ultramicrobacteria which weigh in at about 0.1 picograms each.

There are two alternative solutions that can keep Earth safe. The simplest solution is to presterilize everything before it gets to Earth. The other is to return unsterilized bonus samples of soil, dust and atmosphere for astrobiology to a safe orbit where they can be studied remotely - we can send over 7 tons of instruments in one payload to above G£O on the Ariane 5.

The samples can be sterilized either during the return journey, for instance using nanoscale X-ray emitters, or in a receiving satellite in orbit around Earth using a cobalt 60 source for irradiation with gamma rays or using x-ray emitters, so it is sterilized BEFORE any of it can contact Earth's biosphere. This would achieve virtually the same science especially since the samples will have to be sterilized anyway before they can be distributed to geology labs because they can never pass safety testing.

The best way to sterilize the samples for astrobiology would be to use ionizing radiation. The draft EIS proposes using one megagray of ionizing radiation. This is equivalent to around 10 million years of surface ionizing radiation at 100 milligrays per year (Curiosity measured 76 milligrays a year). This would destroy 12.9% of the amino acids but still leave present day life recognizable.

For an extra safety level, I think 10 Megagrays would be more than enough, as it would destroy 75% of amino acids. At the one in a million survival level, radiodurans can survive destruction of 1.9% of its amino acids. A hypothetical more radioresistant lifeform could survive more. But no matter what it is made of, to break so much of its organic bonds and destroy so many amino acids would surely sterilize it. That's equivalent to only 100 million years of surface ionizing radiation so it wouldn't make much difference to the geological samples unless very recently exposed. We'd still recognize present day life apart from the issue of forward contamination. See technical P.S. at the end of this page for calculations.

If there was concern some hypothetical lifeform could still revive with only 25% of amino acids intact,  50 megagrays would reduce them 1000 fold and still be equivalent to only half a billion years of surface ionizing radiation. Even 50 megagrays equivalent to 500 million years of surface radiation, is still not much compared to the likely billions of years exposure of geological samples unless very recently exposed though it would make the astrobiology harder.

However it's not likely we can do much meaningful astrobiology, with this level of contamination, just more studies to prove in more detail what we know already that Mars was very habitable in the past and that Jezero crater was a river delta three billion years ago.

So, I suggested bonus samples collected in clean containers. We could add samples of dust, dirt and atmosphere to the ESA fetch rover. These also could be presterilized before they get to Earth, and still retain a great deal of astrobiological interest.

For instance we detect microbes in Japan brought there from dust storms in the Gobi desert. Even if there are small amounts of life in distant regions of Mars there is some chance it could be detected in Jezero crater. Maybe even viable life especially if martian life is adapted to spread in the dust storms.

Also Curiosity detected liquid water indirectly in the sand dunes it drove over in Gale crater in the early morning and evening. These are even more likely at Jezero crater where it reaches 100% humidity at night. They have enough water activity for life at -70°C but far too cold at least for terrestrial life but a biofilm could retain the water through to daytime when it rises to above 0°C sometimes.

If there isn’t native martian life there, the salts are still very interesting in clean samples, for prebiotic chemistry and to help with Mars simulation chambers to study what could happen elsewhere on Mars. We could add a small sterile container to the ESA fetch rover to compress the atmosphere, collect dust and as a receptacle for a small scoop of dirt that the rover could dig up and add on top of the sample tubes to return to Earth.

As an alternative to sterilizing the astrobiology samples on the return journey before they get to Earth, we could return these unsterilized bonus samples above GEO (Geostationary Earth Orbit) to a safe orbit while returning all the geological samples presterilized to Earth for the geologists. This is just like several published ideas such as HERRO and the the Lockheed Martin “Stepping Stones” to Mars and Mars Base Camp, all proposals for astronauts exploring Mars telerobotically from orbit to explore Mars at less expense from orbit and safely for both Mars and Earth life before we know what’s there. See:
. HERRO mission to Mars using telerobotic surface exploration from orbit

. Comparison of Deimos and Phobos as destinations for human exploration, and identification of preferred landing sites

. Mars Base Camp: An Architecture for Sending Humans to Mars

Those who want to take special care to protect Earth's environment and humans often suggest returning the samples to a lab run by humans in orbit and protecting Earth by using a quarantine period before they return after handling the samples. However back in the 1970s Carl Sagan already talked about the "vexing question of the latency period" (in 1973, The Cosmic Connection - an Extraterrestrial Perspective) and if you look at it closely, this won't work.

We can't rely on human quarantine, I give many examples in my preprint, e.g. the two Zinnia plants in the ISS which died of a crop opportunistic fungal pathogen Fusarium oxysporum brought there in an astronaut's microbiome. Fusarium oxysporum is also a sometimes deadly pathogen for immunocompromised humans, actually second after Aspergillus in harm to humans for fungi - but obviously had no symptoms with that young healthy astronaut.

Another example I look at is some natural analogue of hachimoji DNA or mirror life independently evolved on Mars. This could be harmless in an astronaut's microbiome but spread and cause major problems in terrestrial biomes once they get back.

If half the life in your forests, soils and oceans is mirror life will it function the same way - after the decades to centuries it might take to adapt to live here?

However, we don't need humans in situ in the lab to do life detection and ultra precise work in astrobiology. Indeed keeping humans out of the facility greatly reduces the risk of forward contamination of the samples - those ultramicrobacteria can go both ways. We can send over 7 tons in one payload to above GEO (Geostationary Earth orbit) already with the Ariane 5 rocket, enough for hundreds of remote life detection instruments in one payload because astrobiology instruments have been so miniaturized in the last couple of decades.. These have automatic sample preparation as there are many such instruments designed for remote operation on Mars.

The draft EIS says, incorrectly, that samples have to come back to Earth for sample preparation. This is needed for some studies yes, but this is NOT required for life detection. There’s even SETG, an end to end gene sequencer which does the sample preparation all the way through to the gene sequencing which requires large amounts of data but it does all the number crunching too and just sends the gene sequence back to Earth.

. SETG: nucleic acid extraction and sequencing for in situ life detection on Mars

I'm no lawyer, and they would need to ask an expert in environmental law, but on the face of it NASA actually may be in some legal jeopardy. By a 7th circuit decision in 1997 it is not permitted to define the scope of an EIS so narrowly as to exclude reasonable alternatives, which the presterilized return certainly is. The remedy back then was that the agency was told to cancel the project to build a reservoir because they improperly excluded the option of two smaller reservoirs. Not because two smaller reservoirs were better, just because they didn't assess that option.

And since we can keep Earth totally safe with virtually no loss of science, why would NASA not even want to consider the pre-sterilized samples option? I just don't get it, I'm totally baffled and have no explanation.

So anyway that's what I wanted to ask you [interested experts] about.

Do you have any idea what is going on? Why NASA aren't responding to me? Is there anything we can do to get answers to this for the general public?

Do feel free to share this email with anyone else. If you know of anyone else I can contact about it also do say.

You can contact me at

I did a blog post and a preprint and an annotated version of the draft EIS that goes into a lot more detail.

You can skip through sections of the blog post with the skip to next section links. I do the articles as like mini abstracts. So you can click through and read the titles of the sections and look at the graphics for each section for a first impression, then drill down into any section of interest. My academic analysis of the draft EIS is done similarly (though with out the skip to next section links) so you can get a good overview of the paper by just reading the contents list then drill into any section of interest. I've also done an annotated copy of the draft EIS which you can look at to see the passages highlighted that I see as of most concern, along with my comments on them. For all this see my:

. NASA - Your Samples From Mars Need A Better Than Biosafety Level 4 Facility - NOT Designed To Contain Even Earth's Tiniest Cells

I hope we can get to the bottom of this. The public surely need answers to their questions and as John Rummel once wrote, which I quote in that blog post:

“Broad acceptance at both lay public and scientific levels is essential to the overall success of this research effort.”

Even if it does go through NEPA, at the end of all the legal process, the president has to look at it, if it can be reasonably expected to result in domestic or foreign allegations of major or protracted effects. So he will have to look at this Environmental Impact Statement carefully, and the current draft EIS will just fall apart on any close study, if they just check the cites, or read my comments on the draft EIS and check them out.

“It should be understood that experiments which by their nature could be reasonably expected to result in domestic or foreign allegations that they might have major or protracted effects on the physical or biological environment or other areas of public or private interest, are to be included under this policy even though the sponsoring agency feels confident that such allegations would in fact prove to be unfounded.

. NSC-25: Scientific or Technological Experiments with Possible Large-Scale Adverse Environmental Effects and Launch of Nuclear Systems into Space

I just want NASA to do it right and it will be a very interesting mission especially if they do the bonus samples for astrobiologists.

Best wishes,


Technical P.S. ionizing radiation levels calculations

According to Kminek et al, The effect of ionizing radiation on the preservation of amino acids on Mars. 100 megagrays is enough to reduce many amino acids to a millionth of the original concentration (500 years at 200 milligrays a year) equivalent to a billion years of surface ionizing radiation at 100 milligrays a year (they assumed higher figures before Curiosity measured them in situ).

Based on 100 megagrays for a million fold reduction, the general formula is

100 / x = log(10^6) / log(n) to get the dose x in megagrays for an n-fold reduction in amino acids

So to get the n-fold reduction given x the formula is n = 10^(log(10^6) / (100/x))

So one megagray will destroy 12.9% of the amino acids calculated as 100 - 100 /(10^(log(10^6) / (100/1)))

Present day life would still be recognizable (apart from the issue of distinguishing it from forward contamination for the sample tubes). If we need more margin, 10 megagrays, equivalent to 100 million years would destroy just short of 75% of amino acids and 5 megagrays equivalent to 50 million years would destroy just short of 50% of them.

From: Effects of Desiccation and Freezing on Microbial Ionizing Radiation Survivability: Considerations for Mars Sample Return. radiodurans is especially hardy when dessicated and frozen and can survive 0.14 megagrays for a reduction of 100,000 to a million fold. Enough to destroy 1.9% of the amino acids. That's less than a sixth of the numbers destroyed at 1 megagray.

Martian life evolved for billions of years in conditions of far higher ionizing radiation than Earth might be able to survive more damage than radiodurans. But even 10 megagrays destroying 75% of amino acids would achieve virtually the same science especially since the samples will have to be sterilized anyway before they can be distributed to geology labs because they can never pass safety testing, and the equivalent of 100 million years of surface ionizing radiation is insignificant for geological samples that have likely been exposed to those conditions for billions of years unless they have evidence they were recently exposed.

See longer version of this post with extra graphics and explanations for non experts.