The state of Rajasthan is situated between 23°3’N and 30°12’ N latitude and 69°30’ and  78°17’  E longitude. The total land area of the state is about 3,42,239 km2, out of which about 1,96,150 km2  is arid and rest is semi-arid. This arid and semi-arid wasteland of is rich in biodiversity. During present investigation studies were conducted on characterization of bio-energy resources in the semi arid region of Rajasthan. 230 plants species were characterized and out of them 60 plant species were selected for dry matter production, 11 plants were characterized for non-edible oil production and 14 plants were   characterized for hydrocarbon production. With the growing demand for fuel wood these plants possibly use as bio-energy sources in their natural habitat.