The Chinese team led by En-Zhi Shen caused a stir amongst experts worldwide. Using a simple measurement in young nematode worms, they reported they had been able to predict how long they would live. Mitochondria are present in most living organisms. They provide the energy for all processes of life and many biologists consider the mitochondria an important biological clock that drives aging. As an underlying cause they suspect that the highly reactive molecules, so called free radicals, released during energy conversion by the power stations can react with cellular molecules causing damage. As a result cellular performance decreases until the cell dies.
This theory is not new – it was first proposed nearly 40 years ago. However, it has not been possible to show a conclusive link between mitochondrial activity, the formation of free radicals and aging.
By introducing a fluorescent probe called cpYFP into those cellular power stations, the mitochondria, of the worms, En-Zhi Shen and colleagues appeared to have found a critical link. They used cpYFP as a free radical detector. And indeed: the more frequently the probe lit up in young worms - the more free radicals they appeared to produce - the shorter the worms lived.
Another team has found that cpYFP is not able to measure free radicals at all. Instead the signals of the probe are the result of changes in pH (that is the acidity) inside the mitochondria.
"From the published worm data we cannot conclude that the degree of free radical release determines lifespan." says Dr. Markus Schwarzländer, research group leader at the University of Bonn and first author of the publication. "cpYFP is not suitable to address this question." He adds that the relationship between the occurance of the probe signals and lifespan of the worms was exciting nevertheless. “Now we can focus on trying to understand its actual significance.“ The new study is soon to appear, also in the journal Nature. 28 experts from 9 countries were involved in this work. It was led by scientists from the University of Bonn, from the German Cancer Research Center Heidelberg, as well as from the Medical Research Council in Cambridge, England.
Citation: Markus Schwarzländer et al.: The ‘mitoflash’ probe cpYFP does not respond to superoxide; Nature Volume 514 Edition 7523; doi: 10.1038/nature13858. Source: University of Bonn.
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