The results give insight into the genetic changes involved in domestication and may help with future efforts to breed new traits into tomato or other crops, said Julin Maloof, professor of plant biology in the College of Biological Sciences at the University of California, Davis and senior author of the study in PNAS.
Breeding new traits into tomatoes often involves crossing them with wild relatives. The new study shows that a large block of genes from one species of wild tomato is present in domestic tomato, and has widespread, unexpected effects across the whole genome. Maloof and colleagues studied the domestic tomato, Solanum lycopersicum, and wild relatives S. pennellii, S. habrochaites and S. pimpinellifolium. Comparison of the plants' genomes shows the effects of evolutionary bottlenecks, Maloof noted -- for example at the original domestication in South America, and later when tomatoes were brought to Europe for cultivation.
Among other findings, genes associated with fruit color showed rapid evolution among domesticated, red-fruited tomatoes and green-fruited wild relatives. And S. pennellii, which lives in desert habitats, had accelerated evolution in genes related to drought tolerance, heat and salinity.
New technology is giving biologists the unprecedented ability to look at all the genes in an organism, not just a select handful. The researchers studied not just the plants' DNA but also the messenger RNA being transcribed from different genes. RNA transcription is the process that transforms information in genes into action. If the DNA sequence is the list of parts for making a tomato plant, the messenger RNA transcripts are the step-by-step instructions.
Gene-expression profiling, combined with an understanding of the plants' biology, allows researchers to understand how genes interact to create complex phenotypes, said Neelima Sinha, professor of plant biology at UC Davis and co-author on the paper. "Genomics has fast-tracked previous gene-by-gene analyses that took us years to complete."
"We could not have done a study like this ten years ago -- certainly not on any kind of reasonable budget," Maloof said. "It opens up a lot of new things we can do as plant scientists."
Citation: Daniel Koenig, José M. Jiménez-Gómez, Seisuke Kimura, Daniel Fulop, Daniel H. Chitwood, Lauren R. Headland, Ravi Kumar, Michael F. Covington, Upendra Kumar Devisetty, An V. Tat, Takayuki Tohge, Anthony Bolger, Korbinian Schneeberger, Stephan Ossowski, Christa Lanz, Guangyan Xiong, Mallorie Taylor-Teeples, Siobhan M. Brady, Markus Pauly, Detlef Weigel, Björn Usadel, Alisdair R. Fernie, Jie Peng, Neelima R. Sinha, and Julin N. Maloof, 'Comparative transcriptomics reveals patterns of selection in domesticated and wild tomato', PNAS 2013, published ahead of print June 26, 2013, doi:10.1073/pnas.1309606110