Before there was life on Earth, there was a primordial soup of molecules, and at some point a specialized molecules began replicating. This self-replication kick-started a biochemical process that would lead to the first organisms.
How those molecules began replicating has been one of science's enduring mysteries.
In the early 1980s, researchers found that ribozymes — RNA enzymes — act as catalysts. It was evidence that RNA can be both the blueprints and the chemical catalysts that put those blueprints into action. This finding led to the "RNA World" hypothesis, which posits that RNA alone triggered the rise of life from a sea of molecules.
But for the hypothesis to be correct, ancient RNA catalysts would have had to copy multiple sets of RNA blueprints nearly as accurately as do modern-day enzymes. That's a hard sell; scientists calculate that it would take much longer than the age of the universe for randomly generated RNA molecules to evolve sufficiently to achieve the modern level of sophistication. Given Earth's age of 4.5 billion years, living systems run entirely by RNA could not have reproduced and evolved either fast or accurately enough to give rise to the vast biological complexity on Earth today.
A paper based on lab experiments during which his team recreated ancient protein enzymes that likely played a vital role in helping create life on Earth flies in the face of the widely-held hypothesis that Ribonucleic Acid (RNA) self-replicated without the aid of simple proteins and eventually led to life as we know it.
"The RNA world hypothesis is extremely unlikely," said Carter. "It would take forever."
There's no proof that such ribozymes even existed billions of years ago but scientists have used 21st century technology to create ribozymes that serve as catalysts. "But most of those synthetic ribozymes," Carter said, "bear little resemblance to anything anyone has ever isolated from a living system."
The authors of the new paper took a different approach. Since our genetic code is translated by two super-families of modern-day enzymes, the research team created and superimposed digital three-dimensional versions of the two super-families to see how their structures aligned. They found that all the enzymes have virtually identical cores that can be extracted to produce "molecular fossils", what they call Urzymes (Ur meaning earliest or original) and the other parts are variations that were introduced later, as evolution unfolded, they say.
These two Urzymes are as close as scientists have gotten to the actual ancient enzymes that would have populated the Earth billions of years ago.
"Once we identified the core part of the enzyme, we cloned it and expressed it," Carter said. "Then we wanted to see if we could stabilize it and determine if it had any biochemical activity." They could and it did.
Both Urzymes are very good at accelerating the two reactions necessary to translate the genetic code.
"Our results suggest that there were very active protein enzymes very early in the generation of life, before there were organisms," Carter said. "And those enzymes were very much like the Urzymes we've made."
The finding also suggests that Urzymes evolved from even simpler ancestors — tiny proteins called peptides. And over time those peptides co-evolved with RNA to give rise to more complex life forms.
In this "Peptide-RNA World" scenario, RNA would have contained the instructions for life while peptides would have accelerated key chemical reactions to carry out those instructions.
"To think that these two Urzymes might have launched protein synthesis before there was life on Earth is totally electrifying," Carter said. "I can't imagine a much more exciting result to be working on, if one is interested in the origin of life."
The study leaves open the question of exactly how those primitive systems managed to replicate themselves — something neither the RNA World hypothesis nor the Peptide-RNA World theory can yet explain. Carter, though, is extending his research to include polymerases — enzymes that actually assemble the RNA molecule. Finding an Urzyme that serves that purpose would help answer that question.
Citation: Li Li, Christopher Francklyn and Charles W. Carter Jr., 'Aminoacylating Urzymes Challenge the RNA World Hypothesis', The Journal of Biological Chemistry, 288, 26856-26863 September 13, 2013 doi: 10.1074/jbc.M113.496125