Researchers have created a programmable DNA thermometer that 20,000 times smaller than a human hair, using a discovery made 60 years ago - that DNA molecules that encode our genetic information can unfold when heated.
"In recent years, biochemists also discovered that biomolecules such as proteins or RNA (a molecule similar to DNA) are employed as nanothermometers in living organisms and report temperature variation by folding or unfolding," says senior author Prof. Alexis Vallée-Bélisle of the University of Montreal. "Inspired by those natural nanothermometers, which are typically 20,000x smaller than a human hair, we have created various DNA structures that can fold and unfold at specifically defined temperatures."
One of the main advantages of using DNA to engineer molecular thermometers is that DNA chemistry is relatively simple and programmable.
"DNA is made from four different monomer molecules called nucleotides: nucleotide A binds weakly to nucleotide T, whereas nucleotide C binds strongly to nucleotide G," explains David Gareau, first author of the study. "Using these simple design rules we are able to create DNA structures that fold and unfold at a specifically desired temperature."
"By adding optical reporters to these DNA structures, we can therefore create 5 nm-wide thermometers that produce an easily detectable signal as a function of temperature," added Arnaud Desrosiers, co-author of this study.
These nanoscale thermometers open many exciting avenues in the emerging field of nanotechnology, and may even help us to better understand molecular biology.
"There are still many unanswered questions in biology," adds Prof. Vallée-Bélisle, "For example, we know that the temperature inside the human body is maintained at 37° C, but we have no idea whether there is a large temperature variation at the nanoscale inside each individual cell."
One question currently under investigation by the research team is to determine whether nanomachines and nanomotors developed by nature over millions years of evolution also overheat when functioning at high rate.
"In the near future, we also envision that these DNA-based nanothermometers may be implement in electronic-based devices in order to monitor local temperature variation at the nanoscale," concludes Vallée-Bélisle.