Risky sexual behavior and substance abuse have always orbited HIV but it isn't just shared needles; drugs like cocaine make people engage in lots of other risky behaviors.
The epidemiology of how HIV spreads is well know but relatively little research has been done into how drugs impact the body's defenses against the virus. A new paper examines how cocaine affects a unique population of immune cells called quiescent CD4 T cells, which are resistant to the virus that causes AIDS.
They found that cocaine makes the cells susceptible to infection with HIV, causing both significant infection and new production of the virus.
For the year long in vitro study, the researchers collected blood from healthy human donors and isolated quiescent CD4 T cells. They exposed the cells to cocaine, then infected them with HIV. They harvested the samples over different time points to trace the cells' susceptibility to infection at different stages of HIV's life cycle, comparing the infected cells with untreated cells.
They found that a three-day exposure to cocaine made the cells more susceptible to HIV infection by stimulating two receptors in the cells, called σ1 and D4. The findings suggest that cocaine use increases the pool of T cells in the human body that can become infected by the virus.
"The surprising result was that the changes cocaine induced on these cells were very minimal, yet they were sufficient to fuel infection," said Dimitrios Vatakis, assistant professor of medicine in the division of hematology/oncology at the David Geffen School of Medicine at UCLA and the study's senior author. "We found that cocaine mediates its effects directly, inducing minimal changes in the physiology of these cells and utilizing the same pathways it uses to target the brain."
As with all in vitro studies, the results may be skewed. Also, they based their research on an acute - that is, brief - cocaine exposure set-up; by contrast, typical drug users are chronic users, meaning that they take the narcotic over extended time. They do, however, have data from their animal models that support and strengthen their observations.
"We have shown that cocaine modulates the permissiveness of quiescent cells to HIV," the researchers conclude. "The potential for cocaine to augment the pool of HIV target cells with a commensurate increase in the viral reservoir has significant implications for HIV seropositive individuals who abuse or use stimulants such as cocaine."
The next stage in the research will be to more closely examine the means by which cocaine makes these once resistant cells susceptible to infection and if the drug does indeed lead to a higher viral reservoir, and to use humanized mouse models to study how drug abuse affects HIV infection as well as the efficacy of Highly Active Antiretroviral Therapy (HAART).
Citation: Sohn G. Kim, James B. Jung, Dhaval Dixit, Robert Rovner, Jr., Jerome A. Zack, Gayle C. Baldwin, and Dimitrios N. Vatakis, 'Cocaine exposure enhances permissiveness of quiescent T cells to HIV infection ', J Leukoc Biol October 2013 94:835-843; doi:10.1189/jlb.1112566