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    First, Second Mouth ...?
    By Robert H Olley | April 12th 2013 05:54 AM | 3 comments | Print | E-mail | Track Comments
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    Until recently, I worked in the Polymer Physics Group of the Physics Department at the University of Reading.

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    Recently, there was found in Spain a shelled Pre-Cambrian Critter which showed distinct evidence of a gut.  This got me thinking about the distinction between the deuterostomes and protostomes, interest in which was sparked again by the even more recent article from National Geographic:

    Giant Sea Cucumber Eats With Its Anus

    And here it is, the giant California Sea Cucumber Parastichopus californicus:



    Now the Sea Cucumber is one group of the Echinoderms, a phylum which along with the Chordates (of which humans make up one genus) and the Hemichordates constitute the Deuterostomes, a select group of creatures in whose embryonic development the first opening to appear, called the blastopore, develop into the anus which therefore appears before the mouth.  As Nicholas Horton so elegantly phrased it: It’s True, We’re all Butt Heads.

    This makes me wonder, what exactly were the feeding habits of the ancestral Deuterostome?  Was it an argument over table manners in the late Ediacaran which propelled the differentiation of the Deuterostomes from all the rest, the Protostomes?

    Invertebrate biology text books tell us little about this.   Although the 1987 Animals Without Backbones: An Introduction to the Invertebrates (Third Edition) by Buchsbaum×2 and Pearse×2 gives a very good introduction to the vastly different embryology of the two groups, the word “deuterostome” hardly gets a look-in, and not much more in Living Invertebrates by the same four authors (same year).

    Was it simply that, while the morphology of the embryos was well understood back in the 60s or before, the actual sequence of their development was only worked out later?  Hardly — a dictionary search shows the word “deuterostome” has appeared in the 50s, and the complexities of development are already apparent in Dynamics in Metazoan Evolution: the Origin of the Coelom and Segments by R.B.Clark, Oxford, 1964, which discusses the differences between deuterostomes, protostomes, and — wait for it — other creatures with three layers of cells.

    Now a possible solution to the mystery is at hand.  Science Nordic has recently regaled us with this delightfully-titled article:

    Anus-mouthed worm looks like our earliest ancestor

    Which refers to Xenoturbella, a very primitive flatworm-like animal found in deep water off the coasts of Scandinavia.  Its very name suggests that it was long thought to be a Turbellarian, a grouping covering all the mostly non-parasitic flatworms, distinct from the exclusively parasitic flukes (Trematodes) and tapeworms (Cestodes).  However, this group has turned out to be something of a catch-all, and has now been broken up into many.

    But Xenoturbella?  As its name suggests, it seems like a rather strange turbellarian.  But recently it was moved to the deuterostomes (on molecular grounds?), even though no-one had actually been able to monitor their development of their eggs until very recently. 

    They can be found by taking hundreds of kilos of mud up from the seabed and then waiting for them to crawl out of the mud. But they are so difficult to keep alive that no-one has succeeded in doing this until now.  The Japanese marine biologist Hiroaki Nakano, of Gothenburg University, Sweden, has not only managed to keep them alive – they have even reproduced,”

    says Peter Funch of Aarhus University.

    They are indeed bilateral, but

    it neither has eyes nor a brain – only a hint of a mouth, and the newly-hatched worms have yet to develop an anus.

    And when an opening does develop, food goes in and out of the one opening.  So if this little critter is hardly changed over 600 million years, it suggests that the other deuterostomes kept the first orifice as an anus, and when a full alimentary canal evolved, food was taken in through the second.




    But back to our sea cucumber.  What about its larval development?  That of the related starfish is quite complicated.  Like all echinoderms, it starts as a bilaterally symmetrical dipleurula, and develops through several forms.  Then a radial adult rudiment forms.  Referring again to Living Invertebrates,
    The final stages of metamorphosis are completed rather rapidly.  A new mouth breaks through on the larval left side through the middle of the ring canal, while a new anus opens on the larval right side, thus producing an adult axis at right angles to the larval axis.  The 5 radial canals grow out and develop tube feet, the body takes on the adult shape, and the young sea star crawls away to a new life.
    This had me somewhat alarmed, so I searched online, without success.  However, in Echinoderms by D Nichols (1962) the larval forms of all the main groups are compared.  The sea urchins follow a path almost a complicated as the starfish, but the sea cucumbers turn out to be somewhat simpler.  The original mouth is retained, but at a certain stage
    The blastopore closes off, but the anus opens again a little way from it, a curious but fairly common phenomenon of development in deuterostomes which is not fully understood.
    So we reach the end of this little journey, but I have a suggestion for a reply to someone whose utterance is only worthy of being flushed into the sewage system:

    Spoken like a true deuterostome!

    Comments

     But if P. californicus were obtaining food via its anus, it would likely use the rete mirabile(a set of blood vessels that connect the sea cucumber’s respiratory trees with its gut) to transfer the food to the gut...To test their idea, the team fed several sea cucumbers radioactive algae, which also contained iron particles. The iron and radioactivity proved an easy way to trace the food as it traveled through the sea cucumber’s body...the researchers also found a high level of radioactivity when they looked at the rete mirabile. The only way that those blood vessels could have such a high concentration of radioactivity is if the animal was transferring food from the respiratory trees to the gut via the rete mirabile.


    The radioactive tags caught my attention. Those have been "traditionally" used to elucidate bio-molecular pathways. To test the idea that the giant sea cucumber was also eating with its anus, Jaeckle and Strathmann used  one-celled algae labeled with 14C. And the "iron particles" were actually iron-containing macromolecules.
    rholley
    iron-containing macromolecules.
    Ferritin and iron dextran, so I read.  So no dragging holothurians full of iron filings with a magnet.

    In looking up the larval development of echinoderms, I found the book by Nichols (1962) most informative, also about the phylum in general.  I particularly like this reconstruction of the Silurian – Devonian Sollasina:




    Robert H. Olley / Quondam Physics Department / University of Reading / England
    I like those old fashioned drawings from natural history texts. When I worked for the Arctic Biological Station a marine biologist had hired an artist on a short-term basis to handle the sketches for his publications. In some ways, I found her work more interesting than some of the tedious stuff the rest of us had to do!