Scientists at Children's Hospital in Pittsburgh have discovered a unique population of adult stem cells derived from human muscle that could be used to treat muscle injuries and diseases such as heart attack and muscular dystrophy.
In a study using human muscle tissue, scientists in Children's Stem Cell Research Center - led by Johnny Huard, PhD, and Bruno Péault, PhD - isolated and characterized stem cells taken from blood vessels (known as myoendothelial cells) that are easily isolated using cell-sorting techniques, proliferate rapidly and can be differentiated in the laboratory into muscle, bone and cartilage cells.
These characteristics may make them ideally suited as a potential therapy for muscle injuries and diseases, according to Drs. Huard and Péault. Results of the study are published in the September issue of the journal Nature Biotechnology.
"Finding this population of stem cells in a human source represents a major breakthrough for us because it brings us much closer to a clinical application of this therapy," said Dr. Huard, the Henry J. Mankin Professor and vice chair for Research in the Department of Orthopaedic Surgery at the University of Pittsburgh School of Medicine. "To make this available as a therapy, we would take a muscle biopsy from a patient with a muscle injury or disease, remove the myoendothelial cells and treat the cells in the lab. The stem cells would then be re-injected into the patient to repair the muscle damage. Because this is an autologous transplant, meaning from the patient to himself, there is not the risk of rejection you would have if you took the stem cells from another source."
Working in dystrophic mice while searching for a cure for Duchenne muscular dystrophy (DMD), Dr. Huard's laboratory team first identified a unique population of muscle-derived stem cells with the ability to repair muscle 8 years ago.
Dr. Péault, a professor in the Department of Pediatrics, Cell Biology and Physiology at the University of Pittsburgh School of Medicine, recognized the importance of determining the origin of these muscle-derived stem cells. His team applied, among others, techniques of confocal microscopy and cell sorting by flow cytometry which led to the discovery in human muscle biopsies that these myoendothelial cells are located adjacent to the walls of blood vessels.
According to their study, myoendothelial cells taken from the blood vessels are much more efficient at forming muscle than other sources of stem cells known as satellite and endothelial cells.
A thousand myoendothelial cells transplanted into the injured skeletal muscle of immunodeficient mice produced, on average, 89 muscle fibers, compared with 9 and 5 muscle fibers for endothelial and satellite cells, respectively. Myoendothelial cells also showed no propensity to form tumors, a concern with other stem cell therapies.
Drs. Huard, Péault and colleagues in Children's Stem Cell Research Center (SCRC) are researching and developing numerous therapeutic uses for the population of stem cells the SCRC team identified. One of the most promising uses could be for the treatment of DMD, a genetic disease estimated to affect one in every 3,500 boys. Patients with DMD lack dystrophin, a protein that gives muscle cells structure.
Dr. Huard is an internationally recognized cell biologist conducting laboratory research into the therapeutic use of stem cells to treat a variety of musculoskeletal and orthopaedic diseases and injuries. In the lab, Dr. Huard is developing cutting-edge therapies to regenerate bone and cartilage and to repair damaged muscle. The application of these therapies could range from the repair of heart muscle damaged by heart attack to the repair of sports-related bone, cartilage and muscle injuries.
Dr. Péault is internationally recognized principally for his work on the prospective identification and characterization of human hematopoietic (blood) stem cells, of which his laboratory has also deciphered the ultimate origin during embryonic life. Besides blood development, his team also is investigating elusive populations of multipotent stem cells that persist in adult tissues, including dispensable ones like fat. Such cells should be invaluable for the regenerative therapy of multiple organs damaged by trauma, aging, genetic or acquired diseases.
- PHYSICAL SCIENCES
- EARTH SCIENCES
- LIFE SCIENCES
- SOCIAL SCIENCES
Subscribe to the newsletter
Stay in touch with the scientific world!
Know Science And Want To Write?
- Gravitational Waves? Watch the LIGO press conference at 10:30 Eastern.
- LIGO, Gravitational Waves, And Laser Interferometry
- Giddings: The 750 GeV Diphoton Resonance Is A Graviton
- The Greenhouse Effect Fallacy
- New Government Guidelines Won't Impact Alcohol Drinking
- Starting Age Of Marijuana Use May Have Long-term Effects On Brain Development
- Internet Searches Reflect Increase In E-Cigarette Popularity
- "Since one direction will be less time in the air (assuming this model is even correct) due to an..."
- "Sadly, given the documented events of his admin., the rule by regulation and the flouting of the..."
- "You may not like my snark, but you haven’t argued with my logic...."
- "After promising 'transparency', Obama has gone down a road of (probably unconstitutional unilateralism)..."
- "Yes the details of the gravitational waves they detect will be very important. Will they..."
- Cotton Candy Cure for Future of Organ Transplants
- Walgreens ‘Selling to Heroin Users’? Yes, to Save Their Lives
- Age-specific Rates Of Dementia In Decline — But Not The Number Of New Patients
- Undoing 35 Years Of Progress? Resistance To AIDS Meds in Africa
- Science Acceptance: The Urban-Rural Divide
- Frying Foods in Olive Oil May Provide Health Benefits