If you want to go on a quest for solving the mysteries of deafness, discovering the genetic machinery in the inner ear that responds to sound waves and converts them into electrical impulses, the language of the brain, is your holy grail.
Scientists at The Scripps Research Institute (TSRI) say have identified just such a chalice; a critical component of this ear-to-brain conversion is the protein called TMHS. This protein is a component of the mechanotransduction channels in the ear, which convert the signals from mechanical sound waves into electrical impulses transmitted to the nervous system.
The key protein they found suggests a promising new approach toward gene therapy. In the laboratory, the scientists were able to place functional TMHS into the sensory cells for sound perception of newborn deaf mice, restoring their function.
"In some forms of human deafness, there may be a way to stick these genes back in and fix the cells after birth," said Ulrich Mueller, PhD, a professor in the Department of Cell Biology and leader of the study.
TMHS appears to be the direct link between the spring-like mechanism in the inner ear that responds to sound and the machinery that shoots electrical signals to the brain. When the protein is missing in mice, these signals are not sent to their brains and they cannot perceive sound.
Specific genetic forms of this protein have previously been found in people with common inherited forms of deafness, and this discovery would seem to be the first explanation for how these genetic variations account for hearing loss.
Many Different Structures
The physical basis for hearing and mechanotransduction involves receptor cells deep in the ear that collect vibrations and convert them into electrical signals that run along nerve fibers to areas in the brain where they are interpreted as sound.
This basic mechanism evolved far back in time, and structures nearly identical to the modern human inner ear have been found in the fossilized remains of dinosaurs that died 120 million years ago. Essentially all mammals today share the same form of inner ear.
What happens in hearing is that mechanical vibration waves traveling from a sound source hit the outer ear, propagate down the ear canal into the middle ear and strike the eardrum. The vibrating eardrum moves a set of delicate bones that communicate the vibrations to a fluid-filled spiral in the inner ear known as the cochlea. When the bones move, they compress a membrane on one side of the cochlea and cause the fluid inside to move.
Inside the cochlea are specialized "hair" cells that have symmetric arrays of extensions known as stereocilia protruding out from their surface. The movement of the fluid inside the cochlea causes the stereocilia to move, and this movement causes proteins known as ion channels to open. The opening of these channels is a signal monitored by sensory neurons surrounding the hair cells, and when those neurons sense some threshold level of stimulation, they fire, communicating electrical signals to the auditory cortex of the brain.
Because hearing involves so many different structures, there are hundreds and hundreds of underlying genes involved—and many ways in which it can be disrupted.
Hair cells form in the inner ear canal long before birth, and people must live with a limited number of them. They never propagate throughout life, and many if not most forms of deafness are associated with defects in hair cells that ultimately lead to their loss. Many genetic forms of deafness emerge when hair cells lack the ability to transduce sound waves into electric signals.
Over the years, Mueller and other scientists have identified dozens of genes linked to hearing loss—some from genetic studies involving deaf people and others from studies in mice, which have inner ears that are remarkably similar to humans.
A Clearer Picture
What has been lacking, however, is a complete mechanistic picture. Scientists have known many of the genes implicated in deafness, but not how they account for the various forms of hearing loss. With the discovery of the relevance of TMHS, however, the picture is becoming clearer.
TMHS turns out to play a role in a molecular complex called the tip link, which several years ago was discovered to cap the stereocilia protruding out of hair cells. These tip links connect the tops of neighboring stereocilia, bundling them together, and when they are missing the hair cells become splayed apart.
But the tip links do more than just maintain the structure of these bundles. They also house some of the machinery crucial for hearing—the proteins that physically receive the force of a sound wave and transduce it into electrical impulses by regulating the activity of ion channels. Previously, Mueller's laboratory identified the molecules that form the tip links, but the ion channels and the molecules that connect the tip link to the ion channels remained elusive. For years, scientists have eagerly sought the exact identity of the proteins responsible for this process, said Mueller.
In their new study, Mueller and his colleagues showed that TMHS is one of the linchpins of this process, where it is a subunit of the ion channel that directly binds to the tip link. When the TMHS protein is missing, otherwise completely normal hair cells lose their ability to send electrical signals.
The scientists demonstrated this using a laboratory technique that emulates hearing with cells in the test tube. Vibrations deflected off the cells mimic sound, and the cells can be probed to see if they can transduce the vibrations in electrical signals—as they would in the body if the cells were then trying to send signals to the brain. What they showed is that without TMHS, this ability disappears.
"We can now start to understand how organisms convert mechanical signals to electrical signals, which are the language of the brain," said Mueller.
Published in Cell