Nuclear reprogramming is changing one kind of cell to that of another unrelated cell type. Techniques have included direct reprogramming, somatic cell nuclear transfer, cell fusion and induction of pluripotency by ectopic gene expression.
Obviously the ability to derive a specialized cell, like a brain cell, from skin tissue would eliminate immunosuppressive issues between genetically different individuals and eliminate ethical issues such as in fields like human embryonic stem cell (hESC) research, which has been limited in federal funding during the Bush and Obama administrations. Researchers including Nobel laureate John Gurdon have written a paper showing that that histone H3.3, deposited by the histone-interacting protein HIRA, is a key step in reverting nuclei to a pluripotent type, capable of being any one of many cell types.
All of an individual's cells have the same DNA, yet as the organism matures these cells become programmed into different types such as heart, or lung or brain. To achieve this, different genes are more or less permanently switched off in each cell lineage. As an embryo grows, after a certain number of divisions, it is no longer possible for cells which have gone down the pathway to become something else. For example heart cells cannot be converted into lung tissue, and muscle cells cannot form bone.
One way to reprogram DNA is to transfer the nucleus of a mature cell into an unfertilized egg. Proteins and other factors inside the egg alter the DNA switching some genes on and other off until it resembles the DNA of a pluripotent cell. However there seem to be some difficulties with this method in completely wiping the cell's 'memory'.
One of the mechanisms regulating the activation of genes is chromatin and in particular histones. DNA is wrapped around histones and alteration in how the DNA is wound changes which genes are available to the cell. In order to understand how nuclear reprogramming works Dr Gurdon's team transplanted a mouse nucleus into a frog oocyte (Xenopus laevis). They added fluorescently tagged histones by microinjection, so that they could see where in the cell and nucleus the these histones collected.
Gurdon explained, "Using real-time microscopy it became apparent that from 10 hours onwards H3.3 (the histone involved with active genes) expressed in the oocyte became incorporated into the transplanted nucleus. When we looked in detail at the gene Oct4, which is known to be involved in making cells pluripotent, we found that H3.3 was incorporated into Oct4, and that this coincided with the onset of transcription from the gene." Prof Gurdon's team also found that Hira, a protein required to incorporate H3.3 into chromatin, was also required for nuclear reprogramming.
Dr Steven Henikoff, of the Fred Hutchinson Cancer Research Center and co-Editor-in-Chief of the journal, commented, "Manipulating the H3.3 pathway may provide a way to completely wipe a cell's 'memory' and produce a truly pluripotent cell. Half a century after showing that cells can be reprogrammed this research provides a link to the work of Shinya Yamanaka (who shared the prize), and suggests that chromatin is a sticking point preventing artificially induced reprogramming being used routinely in the clinic."
Citation: Jerome Jullien, Carolina Astrand, Emmanuelle Szenker, Nigel Garrett, Genevieve Almouzni and John Gurdon, 'HIRA dependent H3.3 deposition is required for transcriptional reprogramming following nuclear transfer to Xenopus oocytes', Epigenetics&Chromatin (in press) (free to read)
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