ABSTRACT
High resolution RP-HPLC method has been developed for the simultaneous estimation of Psuedoephedrine, Fexofenadine and Loratadine in pharmaceutical products. Separation was achieved
by using C18, 150 x 4.6 mm, 5μm column at a temperature of 350C with a gradient mobile phase composed of sol-A: 0.01M pH 3.0 phosphate (KH2PO4) buffer, sol-B: Acetonitrile and gradient
program was 0-5min, sol-A: 80-60; 5-10min- sol-A: 60-20; 10-15min- sol-A: 20-20; 15-17min- sol-A: 20-80 and 17-20min- sol-A: 80-80. Flow rate was 0.60 ml per min and measured the
absorbance at 210nm. The retention time of Psuedoephedrine, Fexofenadine and Loratadine are 4.3min, 10.4min and 17.0min, respectively. The linearity of the proposed method was evaluated
from 5μg per mL to 100μg per mL and the correlation coefficient results were within the limit (not less than 0.999). The developed method has wide applicable for the quantification of
Psuedoephedrine, Fexofenadine and Loratadine in pharmaceutical dosage forms.
Key words: Psuedoephedrine, Fexofenadine, Loratadine, high resolution and RP-HPLC method.