Researchers have linked mutations in specific genes to each of the four recognized subtypes of medulloblastoma, the most common malignant brain tumor of children. The discovery provides doctors with potential biomarkers for guiding and individualizing treatment and reveals prospective therapeutic opportunities for countering this devastating malignancy.
Medulloblastomas occur in the cerebellum, the part of the brain that controls balance and other complex motor functions, and are treated with a combination of surgery, radiation and chemotherapy. Though overall survival hovers around 70 percent, most survivors are unable to live independently due to the lasting effects of both tumor and treatment.
Doctors have historically classified medulloblastoma patients as either standard or high risk based on biopsy results, but have long suspected that what we call medulloblastoma could actually be several different diseases. Over the last two years, studies by researchers including Pomeroy and his colleagues have bolstered this view by dividing medulloblastoma into four molecular subtypes based on gene expression profiles and copy number variations. Each subtype has a distinct survival rate, ranging from 20 to 90 percent.
Functionally, the mutated genes they noted fell into two broad categories: genes like Shh and Wnt that play direct roles in molecular pathways controlling cell growth, and genes like DDX3X and GPS2 that play more of a coaching role, modulating the activity of other genes.
The study was conducted by a research team led by Scott Pomeroy, MD, PhD, Neurologist-in-Chief at Boston Children's Hospital; Yoon-Jae Cho, MD, at Stanford University School of Medicine; and Matthew Meyerson, MD, PhD, of Dana-Farber Cancer Institute and the Broad Institute.
"Not only do we now know how to stratify medulloblastomas genomically, we have a firm grasp of what gene mutations drive each molecular subtype," said Pomeroym, "For the first time, we'll be able to classify and treat medulloblastoma based on molecular typing, providing the best therapy with the fewest long-term consequences."
In this new study, they used sequencing technologies to read the full complement of protein-coding genes, called the exome, of tumors from 92 patients. Within these tumors the team discovered that somatic (that is, non-heritable) mutations occur at very low frequency, one-tenth to one-hundredth of that seen in cancers of adults. Specific gene mutations clustered neatly into the four molecular subtypes, although the majority of genes (88%) were mutated only once in the entire tumor collection. Only 12 genes were mutated in more than one tumor, illustrating medulloblastoma's genetic heterogeneity.
Taken as a whole, the study's results confirm the view of medulloblastoma as a family of tumors driven by disruptions in just a few common mechanisms. However, the form those disruptions take—the actual mutations or genomic changes—can vary from tumor to tumor.
"The results reflect two emerging genetic themes seen throughout childhood tumors," Pomeroy noted. "First, very low mutation rates, much lower than those seen in adult tumors, and second, the importance of mutations in genes that regulate the function of the cell's growth pathways but which aren't direct components of those pathways."
Some of the study's findings could be translated to patients relatively quickly. For instance, with the main mutations of each subtype in hand, it should soon be possible to rapidly classify individual medulloblastoma patients' tumors and tailor treatment appropriately based on each subtype's known prognosis. In addition, clinical trials of Shh-blocking drugs already under investigation for other cancers could begin within the next couple of years in patients with the medulloblastoma subtype driven by Shh mutations.
Pomeroy credits the high level of cooperation between groups at different institutions studying medulloblastoma as a significant factor in the progress made over the last few years. "Because of our collective efforts, medulloblastoma has gone from an important but obscure tumor to one that we understand better than many other cancers at the molecular level."
Published in Nature.
- PHYSICAL SCIENCES
- EARTH SCIENCES
- LIFE SCIENCES
- SOCIAL SCIENCES
Subscribe to the newsletter
Stay in touch with the scientific world!
Know Science And Want To Write?
- Kenya Example: How Re-Analyzing Scientific Research Data Can Change The Findings
- Epigenetics: Quackery Or Phenomenon?
- New Results From The LHC At 13 TeV!
- Kepler 452b - Things That Could Go Wrong With Habitability & If It Is - Could We Detect Intelligent Life There?
- High Z: Metal Foams Shield X-rays, Gamma Rays And Neutron Radiation
- Are All Weight Loss Doctors Quacks?
- "Well, thanks - indeed I have the same impression :) And I do share your feelings about the recruitment..."
- "Exactly - the newest generation of hep C drugs have also drawn a lot of fire, but direct comparisons..."
- "The fervor surrounding the high costs of newcancer medications has many calling for government..."
- "I didn't really misunderstand, but I think what I was pointing out, fits into your system discussion..."
- "I’m not at all surprised by this. The idea that the Greeks used the golden ratio in everything..."
- Very early birth correlated to introversion, neuroticism, and risk aversion in adulthood
- Personality disorders drive psychiatric patients to euthanasia
- Schizophrenia's 'Rosetta Stone' gene identified
- Plasmonic superfast fluorescence sets new speed record
- Residential tourism increased earthquake risk, says sociologist