A breakthrough could speed recovery and limit disfigurement for patients who have suffered large soft tissue trauma, as occurs with serious injury or cancer surgery.
By biomedically engineering a muscle flap that includes a patient's own blood vessels, the team created tissue that could be transferred to other parts of the body along with the patient's blood supply. Current techniques – including grafts and synthetic material – for reconstructing such trauma often fail because of lost blood supply. The scientists fabricated the flap using a variety of added cells and connective tissues to strengthen it. They tested it by reconstructing deep abdominal wall tissue defects in mice.
Successful reconstruction of large, soft tissue defects has been a clinical challenge in the past. To improve outcomes, the researchers developed the muscle flap using a patient's own tissues, added important and advantageous cellular components to strengthen it, and engineered it in such a way as to vascularize to include the patient's own blood vessels so that the patient retained their own blood supply during the reconstruction process.
The study provides evidence that tissue-specific cells, such as myoblasts (cells that form muscles), endothelial cells (the thin layer of cells that lines the interior surface of blood vessels), and fibroblasts (the cells providing the structural framework for animal tissues), are necessary to more effectively integrate within the host tissue.
Within one week of being transferred into the test mice, the engineered muscle flaps were "viable, highly vascularized," and demonstrated "firm attachment to the surrounding tissues." The researchers also noted that the muscle flaps had the mechanical strength to support the "abdominal viscera," or organs in the abdominal region.
The researchers say the results will stimulate more research and lead to clinical studies with human patients. They also suggest there are far-reaching uses of the muscle flap as it can be transferred as a 'free flap' to reconstruct defects in other parts of the body. This could circumvent the need to harvest and transfer large amounts of tissue, avoiding many of the current complications.
"We designed and evaluated an engineered muscle flap with robust vascularization. Proper vascularization is essential for successfully integrating the flap within the host,"said Professor Shulamit Levenberg, of the Department of Biomedical at the Technion-Israel Institute of Technology, who worked in collaboration with Dr. Yulia Shandalov (who was a doctoral student under Levenberg), and Dr. Dana Egozi, from the Rambam Health Care Campus.
Upcoming in the Proceedings of the National Academy of Sciences.
- PHYSICAL SCIENCES
- EARTH SCIENCES
- LIFE SCIENCES
- SOCIAL SCIENCES
Subscribe to the newsletter
Stay in touch with the scientific world!
Know Science And Want To Write?
- Wait, Let's Not Rush To Be Multiplanetary Or Interstellar - A Comment On Elon Musk's Vision
- Bizarre Forelimb Evolution In Ancient Drepanosaurus Fossil
- Ground Squirrels Use The Sun To Hide Food
- Paleo: In A Clinical Trial, Bread Made With Ancient Grains Could Benefit Heart Health
- Study Explains Mechanisms Behind Glioblastoma Influence On The Immune System
- A Book By Guido Tonelli
- 42 Million Years: Central Asia Is Used To Westerly Winds By Now
- "Fantastic article! One thought on the Kardashev scale though, it ignores energy efficiency. It..."
- "So, Irish-catholic ghettos in Belfast in 60ies through 80ies would be a perfect historical example..."
- "Why to fear of nibiru when we know that we all have to die someday Live Big not Long..."
- "Nice piece of information about nibiru i am starting to feel feared..."
- "Right, good thoughts :). On replicators, yes if a species can make robust self replicating spaceships..."
- San Francisco Soda Tax: A Feel-Good Policy Based on Junk Science
- Watson and Crick did not discover DNA
- Is Parenting Kids of Human and Canine Persuasion the Same? Yes!
- Diabetes: MiniMed 670G Hybrid Closed-Loop Insulin System Is A Waypoint To An Artificial Pancreas
- Celebrate Oktoberfest with Beer Chemistry
- Littlest Consumers Doing Well, Nutrition-wise
- 51 U.S. House Members Urges DEA To Delay "Hasty" Ban On Natural Herbal Supplement Kratom
- Women are a quarter of the 1 percent
- Wetlands and agriculture, not fossil fuels, behind the global rise in methane
- Mass immigration is correlated to higher levels of crime, but not causal
- How would you like a kitchen surface that cleans itself?