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    Medicinal plant cultivation.
    By Ashwani Kumar | September 10th 2010 10:54 AM | 4 comments | Print | E-mail | Track Comments
    About Ashwani

    Professor Emeritus ,Former Head of the Department of Botany, and Director Life Sciences, University of Rajasthan, Jaipur. 302004, India At present...

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    Large interest groups get medicinal plants collected from the fields and thus they endanger the plant species in natural conditions and many medicinal plants have come in the category of endangered plants due to their overexploitation. One possible answer could large scale fermenter culture of medicinal plants (Ashwani Kumar, Shikha Roy 2006 Plant Biotechnology and its Applications in Tissue Culture IK international, New Delhi; Karl-Hermann Neumann, Ashwani Kumar and Jafargholi Imani (2009) Plant Cell and Tissue Culture - A Tool in Biotechnology: Basics and Application Springer Heidelberg. Several people have asked through this blog which plants to grow in our area of land . They write they have land, water and money but they do not know which medicinal plants to cultivate for commercial purpose. Mr Arjun Prajapati grows sena patti ( Cassia sena ) among other plants and exports it from Jodhpur. People of Rajasthan collect Aloe vera and send it to Hayrana for processing. Rajasthan has tremendous potential for Aloe vera cultivation. One erstwhile thikanedar ( or small town king ) of Maharaja having enough land cultivated medicinal plants on large scale but he did not find buyers for his products as the roots of ashwgandha did not have enough secondary metabolites. Now following questions must be examined before undertaking medicinal plant cultivation 1. Type of soil, its pH, Ec of soil and irrigation water. 2. Local plants of medicinal value or availability and suitability of exotic plants to local conditions. 3. Proper timing for sowing and harvesting 4. Market potential 5 .Quality control 6. Government regulations for export 7. Management practices. Plant drugs are largely secondary metabolites and their production is inversely proportional to plant growth China and Korea export more crude drugs than India A better management system has to be evolved and international market should be assured of quality. Make survey study for agro technology and botany of medicinal plant before you plunge into cultivation. Medicinal plants are different . Try them they can get you money and reputation both.


    how can I trained up in tissue culture techniqes?

    Ashwani Kumar
    Plant tissue culture technique requres thorough knowledge of theory and it has to be learnt by practically isolating and culturing plant tissues. The following books can help you learn the technique of plant tissue culture and you can chose specific topics from specific books for your requirements of what you want to achieve by tissue culture Literature cited Plant Cell and Tissue Culture - A Tool in Biotechnology Basics and Application Series: Principles and Practice Neumann, Karl-Hermann, Kumar, Ashwani, Imani, Jafargholi 2009, IX, 333 p. 153 illus., Hardcover ISBN: 978-3-540-93882-8 This book provides a general introduction as well as a selected survey of key advances in the fascinating field of plant cell and tissue culture as a tool in biotechnology. After a detailed description of the various basic techniques employed in leading laboratories worldwide, follows an extended account of important applications in, for example, plant propagation, secondary metabolite production and gene technology. Additionally, some chapters are devoted to historical developments in this domain, metabolic aspects, nutrition, growth regulators, differentiation and the development of culture systems. The book will prove useful to both newcomers and specialists, and even "old hands" in tissue culture should find some challenging ideas to think about. Written for: Researchers and advanced students Keywords: plant biotechnology plant breeding plant cell and tissue culture plant genetic engineering Online orders shipping within 2-3 days. Plant Biotechnology and its Applications in Tissue Culture Editor(s): Ashwani Kumar, Shikha Roy ISBN: 8188237507 No. of Pages: 326 Publishing Year: 2006 Binding: Hardback Dimension: 18cm x 24cm Weight: 970 Price: Rs. 1,995.00 $ 133.00 £ 75.00 About the Book: In recent years, plant cells are being increasingly utilized as model systems to unravel the cellular and molecular mysteries of nature. The basic mechanisms of developmental biology, cellular and molecular interactions at the cell and tissue level, gene expression and regulation leading to genetic modifications and areas of genomics, are constant areas of research. The following areas have been highlighted in this book: • Embryogenesis, regeneration and micropropagation • Genomics and genetic transformation systems • Secondary metabolites, metabolic engineering and molecular biology • Resistance to biotic and abiotic stress • Tree biotechnology The present book is an attempt to deal with all the topics in-depth and to find out the molecular aspects of different processes in cultured plant cells, so as to exploit them for h benefit of plant biotechnology. ________________________________________ Contents: Some Studies on Somatic Embryogenesis: A Tool in Plant Biotechnology / Cellular Differentiation and Genetic Transformation Studies in Indian Legumes, Crucifers, Mulberry and Cereals / Agrobacterium Mediated Transformation of Chickpea (cicer arietinum L.) / Constraints and Relvant Strategies in Genetic Transformation of Sorghum / Selection of Transgenic Plants Using Positive Selectable Markers and Other Non-antibiotic Marker Genes / Production of Hybrid Plants in Thompson Seedles Grapes (vitis vinifera) through Breeding and in ovulo Embryo Rescue Methods / Somatic Embryogenesis in Chickpea (cicer arietinum L.) / Polyamines and Long-term Plant Regeneration / Somatic Embryogenesis in Chir Pine (Pinus roxburghii Sarg.) / Molecular Diversity of Elite WA-CMS Maintainer Lines with EUI Gene for Hybrid Breeding in Rice / Characterization of Candidate Clumps of Dendrocalamus Strictus using RAPDs / Plantlet Regeneration from the Nodal Segments of Pomergranate (Punica Granatuk L.) / Hardening of Gracinc indica / Production of plumbagin a multifarious medicinal compund from cultured cells of Plumbago rosea L. / Secondary metabolites form tissue cultures of some arid zone plants / Steroidal sapogenin in moth bean cultivars in vivo and tissue culture / Production of bacoside by multiple shoot cultures and in vitro regenerated plantlets of slected cultivar of Bacopa monnieri (L) Wettst / Biotechnology medicinal plants / Somaclonal variants for salt tolerance and in vitro propagation of peanuts / Flow cytometry: A comparatively new tool in plant biotechnology / Steroid factories: Prospects and practicalities in effective farming of Solanaum plants / Genetic Transformation of grain legumes / In vitro Adventitive Embryony: A safe alternative to Citrus budwood importation / Micropropagation of Gmelina arborea Roxb. Using mature tree tissues – Optimization of in vitro responses / Challenges in cashew tissue culture: an overview Recent Advances in Plant Biotechnology and its Applications Editor(s): Ashwani Kumar, Sudhir K. Sopory ISBN: 8189866095 No. of Pages: 718 Publishing Year: 2008 Binding: Hardback Dimension: 18cm x 25cm Weight: 1805 Price: Rs. 2,495.00 $ 175.00 £ 99.00 Recent Advances in Plant Biotechnology and its Applications Editor(s): Ashwani Kumar, Sudhir K. Sopory ISBN: 8189866095 No. of Pages: 718 Publishing Year: 2008 Binding: Hardback Dimension: 18cm x 25cm Weight: 1805 Subject: Biotechnology Price: Rs 2,495.00 $ 175.00 £ 99.00 ________________________________________ About the Book: The present book is divided into five sections. The first section deals with the methodology and bioresource generation, techniques related to genetic engineering, and gene transfer to the nuclear genome and chloroplast genome. The new techniques of genome profiling and gene silencing are also presented. The second section of the book deals with the classical aspect of plant biotechnology viz. tissue culture and micropropagation. Use of genetic engineering via Agrobacterium and direct transfer of DNA via particle bombardment to develop transformed plants in Artemesia, castor and orchids, and production of recombinant proteins in plant cells have been dealt with in the third section. The fourth section deals with the abiotic and biotic stress tolerance in plants. The basic biology of some of the stress responses, and designing plants for stress tolerance is discussed in this section. The fifth section deals with medicinal plants and alkaloid production. ________________________________________ Contents: SECTION 1: METHODOLOGY: Wild Species of Oryza as an Important Reservoir for Mining of Useful Alleles / Plant Genetic Transformation: Methods and Emerging Trends / The Engineering of Recombinant Plastids in Higher Plants / Inducible Gene Expression Systems as Tools for Plant Functional Genomics / Gene Targeting in Higher Plants / State-of-the-art Genome Profiling (GP): A Key Tool for Plant Biotechnology / Applications of Gene Silencing in Crops SECTION 2: PHYSIOLOGY, TISSUE CULTURE, MICROPROPAGATION: Effect of Brassinolide on Growth, Yield, Metabolite Content and Enzyme Activities of Tomato (Lycopersicon esculentum Mill.) / Ornamental Foliage Plants: Improvement through Biotechnology / In vitro Propagation of Tropical and Subtropical Fruit Crops / In vitro Plantlet Regeneration in Asparagus racemosus through Shoot Bud Differentiation on Nodal Segments / Micropropagation of Ringal Bamboo Drepanostachyum falcatum (Nees) Keng. F. through Nodal Segments / Isolated Microspore Embryogenesis in Cereals: Aspects and Prospects / Plant Regeneration from Cell Suspension Culture-Derived Protoplasts of Phellodendron amurense Rupr. / Importance of Micropropagation Technique in Enhancing the Productivity of Crops SECTION 3: PLANT TRANSFORMATION: High Efficiency Transformation in Artemisia annua with Agrobacterium tumefaciens / Genetic Improvement of Castor (Ricinus communis L.) through Tissue Culture and Biotechnological Tools / Genetic Engineering of Orchids / Identity Preservation in Genetically Modified Crops / Enhanced Production of Recombinant Proteins from Plant Cells / Bioengineering of Crops for Biofuels and Bioenergy/ SECTION 4: STRESS TOLERANCE: Light and Dark Modulation of Chlorophyll Biosynthetic Genes in Response to Temperature / Glutathione Homeostasis and Abiotic Stresses in Plants: Physiological, Biochemical and Molecular Approaches / Signaling Components in Programmed Cell Death: Molecular and Functional Similarities between Animals and Plants / Regulation of Stomatal Opening and Signal Transduction Components in Guard Cells / Designing New Plant Genotypes with Enhanced Resistance to Salinity / Defense Response of Moth Bean (Phaseolus aconitifolius) to Macrophomina phaseolina: Role of Polyphenols, PAL and β-1,3-glucanase / Host-Pathogen Interaction in Sesamum indicum: Synthesis of PR-Proteins / Allium sativum Leaf Lectin: A Potent Sap Feeding Insect Control Protein Expressed in Rice and Mustard / Genetic Engineering: A Powerful Tool to Combat Viral Diseases of Plants SECTION 5: BIOTECHNOLOGY OF MEDICINAL PLANTS: Molecular Tools in Medicinal Plants: A Review / Forskolin Biosynthesis and Invertase Activity in Agrobacterium rhizogenes Mediated Transformed Roots of Coleus forskohlii / A Novel Source for Furanocoumarins: Ruta graveolens L./ Antioxidant Potential of Plants and their Impact on Human Health / Arabinogalactan and Arabinogalactan-Protein Complexes (AGPs): a Natural Plant Derived Macromolecule of Therapeutic Importance / Transgenics of Some Medicinal Plants / In vitro Regeneration of a Rare Medicinally Potent Plant of Eastern Himalayan Hotspot / A Comparative Study of Growth Kinetics in Hairy Root Cultures of Solanum khasianum Clark Grown in Shake Flasks and Bioreactors / Micropropagation and Alkaloid Production through Somatic Embryo Cultures of Corydalis / Biotechnological Approach on Production of Ginkgolides from Cell Cultures of Ginkgo biloba L./ Applications of Biotechnology in Indian Ginseng (Ashwagandha): Progress and prospects / Prospects of Ephedrine in the Desert Plants of Rajasthan / Ayurvedic Medicines : Some Potential Plants for Medicine from India / Index Ashwani Kumar, Professor, Department of Botany, University of Rajasthan, Jaipur, has earned gold medal in M.Sc, and obtained Ph.D. from Rajasthan University. He also has the distinction of being an Alexander Von Humboldt Fellow (Germany). He was the Honorary Director at the School of Life Sciences, University of Rajasthan. The author’s repertoire of published works spreads across 150 research articles in various national and international journals. With an experience of over three decades in his field of research, namely, tissue culture and biochemistry, the author has been at the helm of various major projects carried out by USDA-ICAR, U.G.C., M.N.E.S, C.S.I.R and DBT. He has long association with Professor Karl-Hermann Neumann and subsequently with Professor Dr Sven Schubert at Institut für Pflanzenernährung, Justus Liebig Universität, Giessen, Germany, as visiting Humboldt Fellow and visiting Professor at Toyama Medical and Pharmaceutical University, Toyama, Japan. The author is currently engaged in research on salinity tolerance at molecular level in cereals and crops. Sudhir K Sopory, Head of Plant Molecular Biology Division at the International Centre for Genetic Engineering and Biotechnology, New Delhi, is a well-known Plant Biologist who is recognized for his contributions in research and teaching in the field of Molecular Plant Physiology and Plant Biotechnology. He obtained Ph.D. from the University of Delhi. He is a visiting scientist at Max-Planck-Institute, Kolen, and University of Munich, in Germany, and at the University of Texas, Austin and United States Department of Agriculture, Maryland, USA. Prof. Sopory is one of the pioneers in the country for adapting genetic engineering technologies for the improvement of plant productivity. He has published over 175 papers and over 50 book chapters and co-edited 8 books. Prof. Sopory has received numerous awards and distinctions including Chakravorty Award, Indian Science Congress, 1986 (given by the Vice-President of India); Shanti Swaroop Bhatnagar Award, (CSIR) 1987 (given by The Prime Minister of India); Fellow, Indian Academy of Sciences, Bangalore, 1992; Fellow, Indian National Science Academy, Delhi, 1992; Fellow, National Academy of Sciences, Allahabad, 1994; Birbal Sahni Medal, Indian Botanical Society, 2001; Fellow, National Academy of Agricultural Sciences, 2002; Tenth Godnev Award lecture of the Belarus Academy of Sciences, 2003; Vice-President, Indian National Science Academy, 2004-2006; Birbal Sahni Centernary Gold Medal Award for Life Time Achievement in Plant Sciences, Indian Science Congress Association, 2005 (given by the Prime Minister of India); Fellow: The World Academy of Sciences (Trieste), 2005; Padmashree, 2007, given by the President of India. --------------------------------------------------------------------------------------------------------- Plant Tissue Culture and Molecular Markers: Their Role in Improving Crop Productivity Editor(s): Ashwani Kumar, N.S. Shekhawat ISBN: 8189866105 No. of Pages: 688 Publishing Year: 2009 Binding: Hardback Dimension: 18cm x 25cm Weight: 1645 Price: Rs. 2,495.00 $ 220.00 £ 115.00 About the Book: Plant tissue culture techniques help in understanding basic life processes and their proper understanding is essential for improving crop productivity. Besides, recently molecular biology has assumed great importance with respect to plant biotechnology. The present book amalgamates all three aspects into one, practical applications of various techniques being the need of the hour. It discusses micropropagation studies on several crop plants, molecular basis of understanding various life processes including molecular basis of somatic embryogenesis and other physiological and biochemical processes having significant biotechnological applications. It also includes in vitro studies of some important plants like Aloe vera, Simmondsia chinensis, Anacyclus pyrethrum and Crataeva nurvala, Arachis hypogaea L., Phoenix dactylifera, Dendrocalamus asper, Asparagus adescendens Roxb., natural products of plant origin with their therapeutic potential and biotechnological production, genome analysis of crop plants with future applications in biotechnology etc. ________________________________________ Contents: Contamination Control and Enhanced Axillary Budding from Mature Explants of Taxus baccata ssp. Wallichiana / Bioinformatics: an Overview / Intellectual Property Rights in Biotechnology / In Vivo and In Vitro Studies on Plant Tumors / Integrated Approaches of Mutagenesis and In Vitro Selection for Crop Improvement / Clonal Fidelity in Micropropagated Plants / Tissue Culture of Date Palm (Phoenix Dactylifera L.)—A Non-conventional Approach / Cytological Analysis of Nodal Stem Segment and Callus Regenerated Plantlets of Azadirachta indica A. Juss. (Neem) / Establishment of an in vitro Clonal Propagation Method for Commercial Exploitation from A High Biomass Yielding Aloe Vera (L.) Germplasm / Unravelling the Molecular Basis of Somatic Embryogenesis / Doubled Haploids in Crop Brassicas: Retrospect and Prospects / A Review of Tissue Culture Studies on Bamboos / Cloning of Female Plant of Simmondsia Chinensis (Link) Schneider Selected for Cultivation in Rajasthan / Comparative Studies on Somatic Embryogenesis and Plant Regeneration from Immature and Mature Zygotic Embryo Axes of Groundnut (Arachis hypogaea L.) / In Vitro Studies and Micropropagation of Anacyclus pyrethrum and Crataeva nurvala / Micropropagation and Large-Scale Plant Regeneration of Edible Bamboo Dendrocalamus asper through Somatic Embryogenesis / Natural Products of Plant Origin with Therapeutic Potentiality and their Biotechnological Production / Micropropagation of Medicinal Plants of Indian Desert and Aravallies / Morphological and Biochemical Changes in in vitro Raised Arachis Hypogaea Var RG-141, Under Different Growth Regulators / Morphogenesis in Cultured Tissues of Three Grass Species / In Vitro Propagation of Vigna sp / Biology and Biotechnology of Groundnut (Arachis hypogaea L.) — A Review / Propagation of Chlorophytum Borivilianum San. and Fern.—A Review / Recent Advances in Micropropagation of Chlorophytum Borivilianum Sant. & Fern. / Enhancement of Secondary Metabolites by Elicitation and Metabolic Engineering / High Frequency Plant Regeneration for Saccharum Munja — A Useful Plant of Arid Area / Biology and Biotechnology of Lasiurus Sindicus (Shewan): A Valuable Grass of Thar Desert / Liquid Culture System Stimulates In Vitro Growth and Shoot Multiplication in Some Medicinally Important Plant Species of Aravallis in Rajasthan / In Vitro Approaches for Plant Regeneration and Conservation of Some Potential Medicinal Plants / Rejuvenation of Mature Plant Tissues / Molecular Characterization of Diversity in Feronia limonia Using ISSR Markers / Molecular and Morphometric Diversity in Relation to Breeding System in Andrographis Paniculata (Burm. f.) Nees / An Approach to Determine Polymorphic and Sharing of Genome Between Crops of Poaceae Family (Wheat, Rice and Sugarcane) Using RAPD as Genetic Tool / Molecular Biology of Fruit Ripening / Molecular Markers — an Important and Indispensable Biotechnological Tool for Crop Improvement and Conservation / Morphological and Molecular Characterization of a Rare Medicinal Herb ‘Safed Musli’ (Chlorophytum Borivilianum Sant. Et Fernand.) / Biochemical Changes Associated with Morphogenesis in Saffron / Insecticidal Genes and their Sustainable Use in Insect Resistant Transgenic Crops / Gene Tagging : A Golden Research Area in Agriculture Biotechnology / Alternative Respiration may Have a Role in Reducing Oxidative Stress in Plants / Tissue Culture Techniques in Introgression of Resistance to Alternaria helianthi from Wild Sunflowers to Cultivated Sunflower (Helianthus annuus L.) / Virus Resistance Inducing Proteins From Plants / Effect of Nacl and Thermal Stress on Proline Content and Electrophoretic Behaviour of Protein in Small-Millets Seedlings Grown In Vitro / A Study of Systemic Acquired Responses to Pathogen Attack in Certain Economically Important Crops / Efficient Microdosing of Phosphorus to Pearl millet Hybrids (Pennisetum americanum L.) for Improved Seedling Establishment under Nutrient-Stressed Environments / Index Readership: The book is useful for postgraduates and researchers in plant biotechnology and related areas like plant science, biotechnology, bioscience, environmental science, food biosciences and chemistry. It will also be of interest for professionals working in the plant biotechnology industry.
    hello sir,
    I am interested in extracting herbal plant secondary metabolites for my research purpose.there is two problem regarding this first of all in different paper for same metabolites different quantitative method is followed so, I am bit confuse.Moreover, i am not understanding after extracting how can i bring my product to market..I want to work for human welfare..

    Ashwani Kumar
    Cultivation of medicinal plants, harvesting at right age, and finding market for the products is extremely tricky. Its not science alone Its commerce too.