Metforin, the most commonly prescribed diabetes drug, kills tumor cells that lack key regulatory gene p53, according to a new study by researchers at the University of Pennsylvania School of Medicinr.
More than half of all human cancers have lost the p53 gene. Yet even in an era of molecularly targeted therapies scientists have had trouble figuring out how to compensate for the absence of a gene. Unlike a genetic mutation that changes the function or activity of a gene, which can be inhibited by a well-tailored drug, loss of a gene leaves nothing for the drug to target.
“This is the first time you can show that tumor growth is impaired by a diabetes drug,” says senior author Craig B. Thompson, MD, Director of the Abramson Cancer Center and Chairman and Professor of Cancer Biology and Medicine. “It is specific for tumors that lack p53, which is the most common mutation in human cancer.”
Thompson and his team, however, have been accumulating evidence over the last several years that p53, best known as a regulator of cell division, controls several metabolic pathways in cells. For potential cancer therapies, that means a drug that affects pathways controlled by p53 could help control p53-deficient tumors.
Significantly, the regulation of metabolic pathways by p53 is also influenced by metformin, the most widely used diabetes drug. Metformin activates the metabolic enzyme AMPK (AMP activated protein kinase), which exerts changes on cellular metabolism by affecting p53 function. Two observational studies already show that diabetic patients who take metformin have a lower rate of cancer diagnosis and mortality than other diabetics.
Thompson’s group hypothesized that metformin may specifically slow the growth of cancers that lack p53. To find out, they injected human colon cancer cells that have normal p53 function into one side of mice and colon cancer cells that lack p53 into the other side. Four days later they started treating the animals with a daily injection of either a saline control solution or with metformin, using a dose comparable to diabetic treatment in humans.
Four weeks later, the p53-deficient tumors in mice treated with metformin were half the size of the p53 deficient tumors in control mice. There was no difference in the size of the p53 normal tumors between the animals treated with metformin or saline. They concluded that metformin slowed the growth of the colon cancer cells that lack a normal p53 function.
The researchers found that metformin instructs cells to switch metabolic pathways. Instead of using the most energy efficient pathway – called oxidative phosphorylation – the cells are forced to use stress-related ones, which are typically used when the cell is short on oxygen, glucose or other nutrient sources. But in the absence of p53, the cells can’t make the switch. “Without p53, if we force cells to live on alternative substrates, they can’t do it,” explains Thompson.
Thompson’s team is now working with collaborators to decide how best to translate these novel observations into clinical practice. If preclinical tests continue to look promising, development of metformin as a cancer therapy may move quickly as the drug is already approved by U.S. Food and Drug Administration for use in humans, the researchers surmise.
The study was funded by grants from the National Cancer Institute.
Monica Buzzai, a graduate student in Thompson’s lab, is first author on the study. Co-authors include Russell Jones, Julian Lum, and Ralph DeBerardinis from Penn; Ravi Amaravadi and Fangping Zhao from Penn and The Children’s Hospital of Philadelphia; and Benoit Viollet from the Centre National de la Recherche Scientifique and Inserm.
Source: University of Pennsylvania School of Medicine
- PHYSICAL SCIENCES
- EARTH SCIENCES
- LIFE SCIENCES
- SOCIAL SCIENCES
Subscribe to the newsletter
Stay in touch with the scientific world!
Know Science And Want To Write?
- Could A Star Orbit A Planet? - Just For Fun
- Supertranslations And Eternal Ghosts: Black Holes No Closer To Being Understood
- Bel's Temple in Palmyra Is No More
- Blade Runner: The Science In The Fiction
- Highlights From ICNFP 2015
- If Happy Meal Law Passes, NYC Kids Will Frown
- An Historical Moment For Diabetes
- "Academics do not keep their jobs according to how many people see their FB posts! Publications..."
- "Joe Mercola is not a mature counterbalance to anything. He is a fear-promoting merchant of doubt..."
- "That took a long time. The investment was worth it though. The story feels logically consistent..."
- "True, because there are no objective standards, and high impact factor science journals are not..."
- "I should say that I am pissed off, and at the same time I feel ashamed as I do seem to care more..."
- Relativistic Heavy Ion Collider confirms tiny drops of early universe 'perfect' fluid
- Magnetic fields provide a new way to communicate wirelessly
- With tobacco, what you don't know can kill you sooner
- Isthminia panamensis: New species of ancient river dolphin discovered
- Smoking prevalence stays the same but people who want to quit are up